Investigation Of Osteogenic Effects About Ca-P Biomimetic Coatings Including Simvastatin On Pure Ti Surfaces

Materials and Methods:In today's studies,we prepare simvastatin solustion with different concentration that be add into simulated body fluids which can be co-deposited onto the surface of titanium plates. By scanning electron microscope (SEM), X-ray diffraction apparatus(XRD) and fourier transform infrared spectroscopy(FTIR),we can observe the micostructure.In vitro,we can gain its influence on MEC3T3-E1 cells by detecting the alkaline phosphatase(ALP) activity.Results:It is observed that in low-dose group,drugs had not only influenced the macrostructure of Ca-P coatings, but also became one part of Ca-P crystals.In vitro, MC3T3-E1 cells were inoculated onto the surface of biomimetic coatings in4,7 and 14 days, we found drugs could be released from the coatings and induce MC3T3-E1 cells differentiation in all groups especially in 1uM groups by alkaline phosphatase(ALP) detection.Conclusion:We can conclude that biomimetic means is the most effective way to incorporate drugs into surfaces of implants without influencing drug's characteristics and make them released sustainedly to produce a marked effect.Meanwhile,it is found that low-dose drugs are more effective and safer than high-dose ones.