| Objective:To investigate the adsorption behavior of bovine serum albumin(BSA) on calcium phosphate/chitosan(Ca P/CS) composite coating.Methods: CaP coating, CS coating and CaP/CS complosite coating were formed on the Ti sensor surfaces by electrochemical deposition, respectively. These coatings were characterized by scanning electron microscope(SEM), energy dispersive X-ray spectroscopy(EDS), and fourier transform infrared spectroscopy(FTIR). BSA were then adsorbed on the four kinds of surfaces under different concentration(0.1, 0.3, 0.5, 0.7, 0.9 mg/m L), respectively. The changes of frequency and mass were determined by QCM-D. The conformation changes of BSA on four kinds of surfaces were characterized by FTIR. The amounts of absorbed BSA on four kinds of surfaces were statistical analysed by one-way ANOVA and SNK-q test.Results: SEM, EDS, and FTIR data showed a uniform Ca P coating, CS coating and Ca P/CS composite coating were built up on the Ti sensor surface by eletrochemical deposition. BSA absorption to four kinds of surface followed Langmuir mechanism. Ca P/CS composite coating displayed the most BSA absorption amount, followed by Ca P coating, CS coating, Ti surface(P < 0.05). FTIR showed that the BSA presented β-turn as main second structure after adsorbing to Ti surfaces, whereas it showed α-helix structure as main second structure on other three kinds of surfaces.Conclusion: Compared with Ca P coating, CS coating and Ti surfaces, the Ca P/CS composite coating have most affinity for BSA absorption under same conditons. The α-helix structure of BSA adsorption on Ca P/CS composite coating may help osteoblasts biological mineralization process in vivo. |
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