The Mechanism Of MicroRNA-106b On Human Bladder Ancer BIU-7Cells

Objectives：Explore microRNA-106b on human bladder cancer BIU-87cellproliferation, apoptosis, and invasion ability to provide the theoretical basis andexperimental evidence for microRNA-106b used in clinical diagnosis and treatment ofbladder cancer.Methods：Chemically synthesis miRNA-AS-106b, and then transfected it intoBIU-87cells transiently using flow cytometer (FCM) tests the effective. After that,use Real-time Quantitative polymerase chain reaction (QRT-PCR) to detect therelative expression of microRNA-106b in BIU-87cells. Make sure that the BIU-87cells had been successfully transfected and on a low rate. Proliferation, invasion andapoptosis of the BIU-87cells were respectively evaluated by MTT experiment,transwell invasion assay and flow cytometer (FCM) test.Results：1、microRNA-106b has a lower expression level：The bladder cancerBIU-87cells are transfected by AS-miRNA-106b and then used Real-timeQuantitative polymerase chain reaction(QRT-PCR) to detect microRNA-106b, wefind that the relative content was lower than the others (P<0.05).2﹑Cell proliferationwas inhibited：Compare transfected AS-miRNA-106b group with the control groupand the blank one that the AS-miRNA-106b group could significantly inhibit cellproliferation (P<0.01).3、Reduced cell invasion：In the transwell invasion assay，we compared the experimental group (19.00+1.41) with the control group(27.25+6.42)(P<0.05) and the blank one (30.75+4.99)(P<0.01) that theAS-miRNA-106b could significantly inhibit cell invasion of the bladder cancerBIU-87cells.4﹑Higher rate of apoptosis：In the FCM test，we put each group intothe flow cytometric and found that the apoptosis rates of the experimental group, thecontrol group and the blank group respectively was (12.08±0.66)%,(5.42±0.11)%and (2.81±0.16)%. The apoptosis of bladder cancer BIU-87cells was obviouslyincreased (P<0.01). Conclusion：Bladder cancer BIU-87cells transfected with AS-miRNA-106bmicroRNA-106b were downregulated. The lower expression rate of microRNA-106bsignificantly suppressed proliferation force and invasion power of BIU-87cells, andcan accelerate BIU-87cells apoptosis. Therefore, microRNA-106b may be involvedin some important variation of biological behaviour in the bladder cancer BIU-87cells. Suppressing the expression of miRNA-106b is expected to become an effectivemethod in gene therapy of bladder cancer.