Preliminary Research On The Proliferation Function Of MiR-106b-5p In Melanoma

Research ObjectivesMalignant melanoma is a class of disease caused by multiple factors. Our study aimed to confirm proliferation functions of miR-106b-5p in melanoma, and provide a scientific basis for further study of the molecular mechanism of melanoma.MethodsApplying real-time PCR technology to detect the miR-106b-5p expression in melanoma and benign nevi tissue samples, and then verifying results in cell lines by RT-PCR. Establishing A2058 cell line downexpressing miR-106b-5p by infection of miRNA inhibitor. Conducting a series of in vitro experiments, including MTS cell proliferation assay, plate clone formation assay, flow cytometry cell cycle and apoptosis analysis, transwell etc. to evaluate the effects of miR-106b-5p on melanoma.ResultsWe conducted real-time PCR analysis in melanoma and benign nevi tissue samples and we found that miR-106b-5p was highly expressed in melanoma samples relative to benign nevi samples. Futher more, we found miR-106b-5p was expressed in melanoma cell lines. MTS and colony formation assay showed that miR-106b-5p promoted cell proliferation and colony formation. Flow cytometry cell cycle analysis evaluated that melanoma cell line had an increased proportion of G0/G1 phase after infection of miRNA inhibitor. Flow cytometry apoptosis analysis showed that knockdown miR-106b-5p had no effect on cell apoptosis. And based on our results, miR-106b-5p had no effect on melanoma metastasis.ConclusionsThese findings demonstrated that miR-106b-5p was highly expressed in melanoma, may stimulate cell proliferation of melanoma.