MicroRNA-106b Regulate Cell Growth, Migration And Invasion In Non-small Cell Lung Cancer A549 Cell

Objective In this study, the aim of the present study was to investigate the role of mi R-106 b in lung cancer and the potential predictive value of mi R-106 b in postoperative NSCLC patients.Methods Compared with their matched adjacent normal tissues and normal cell line, mi R-106 b was significantly overexpressed in human NSCLC tissues and cell line by quantitative real-time PCR. Moreover, through MTT assay, Mimic-mediated elevation of mi R-106 b could markedly promote the growth of NSCLC cells, and overexpression of mi R-106 b significantly promoted invasion and migration by transwell. While mi R-106 b inhibitor played an opposit role in NSCLC cells. Furthermore, using luciferase reporter assay and western blotting, we confirmed that mi R-106 b could directly target the 3'-UTR of PTEN in NSCLC cells and negatively regulate the expression of PTEN m RNA and protein, vice versa.Results In the current study, we investigated that overexpression of miR-106 b was found in NSCLC tissue samples and cells and resulted in downregulation of PTEN. Moreover we found that mi R-106 b promote NSCLC cells growth, migration, invasion and reduced apoptosis. We confirmed that mi R-106 b could directly target the 3'-UTR of PTEN in NSCLC cells and negatively regulate the expression of PTEN m RNA and protein, vice versa.Conclusion Our results demonstrated that mi R-106 b was involved in tumorigenesis and may regulate the cell growth, migration and invasion by directly targeting PTEN in NSCLC. It may be a novel potential therapeutic target of NSCLC.