The Role Of Serum MiR-19b、 MiR-29c In Nasopharyngeal Carcinoma Diagnosis And The Preliminary Searching About Molecule Pathaway

Background: Nasopharyngeal carcinoma (NPC) is a malignant tumorwhich is concerned with EB virus infection, multiple genes involved,genetic and environmental factors in synergy. It had a Higher incidence inthe province of Guang Dong、Guang Xi and Fu Jian. There is a largerdifference between male and female, the incidence of the disease frommale is more than twice times than female. MicroRNAs (miRNAs) isnon-coding Small molecule RNA which can play a important regulatoryroles in animals and plants. It can inhibit the translation of protein ordegrading the target mRNA through matching with it. The tumor is closely related to the location of miRNA in chromosome. It has beenshown that miRNAs undergo the regulatory mechanism which calledepigenetic regulation. Epigenetic regulation can inhibit the oncogene bymaking the gene which have expressed silently re-expressed. MiRNA hadthe role of oncogenes and tumor suppress genes depend on its expression,so it is closely related to the tumor. In the recent years, we found thatthere were several miRNAs existed in the serum and plasma, furthermorethese miRNAs have certain characteristics: first, the serum miRNAs hada stability of anti-RNA Nase, acid resistant, alkali resistant andanti-freeze-thaw. Second the serum miRNAs expression were consistentin normal while there was a big difference between different tumors,and the character of tumor and prognosis were highly depended on thedifferent expression of miRNA. So, miRNA had the favorable conditionto be a biomarker for tumor diagnosis. The scientists have detected theprofile of serum miRNA and found that there were many expresseddifferently miRNA exist in the NPC. For example, the expression ofmiR-29c declined5times compared with normal, and our team hadresearched that miR-19b have risen more than10times in NPC cellwhich was positive expressed LMP1. At present, there are severalpredicts about miRNA target gene, but the mechanism is still not clear.Objective:(1) The aim of the study was to discuss the significance ofmiR-19b and miR-29c as a potential clinical diagnosis biomarker for NPC by detecting their different expression in NPC、 NPC afterchemo-radiotherapy and non-tumor, and discuss their significance as abiomarker for monitoring the recurrence of NPC after treatment.(2)Weidentified the different expression of SOCS-1and COL1A1which are thetarget gene of miR-19b and miR-29c by immunohistochemistry betweenNPC and nasopharyngeal mucosal chronic inflammation. A preliminarysearching about the molecular pathway in canceration will be given bythe relative analysis of miR-19b、miR-29c and SOCS-1、COL1A1. It willgive a reference for the NPC target gene therapy.Methods:30NPC、30NPC after chemo-radiotherapy and30non-tumor human serum were collected first, and then analyse thedifferent expression of miR-19b and miR-29c by by RNA extraction、reverse transcription、and qRT-PCR.(2)30tissue wax block of NPCwhich have been collected serum were gathered, and we gathered30tissue wax block of nasopharyngeal mucosal chronic inflammation, weidentified the different expression of SOCS-1and COL1A1byimmunohistochemistry. A preliminary searching about the molecularpathway in canceration will be given by the relative analysis of miR-19b、miR-29c and SOCS-1、COL1A1.Results: compared with non-tumor person, we find that there was aclearly risen tendency of miR-19b and miR-29c in NPC by qRT-PCR. Weidentified that the expression of miR-19b and miR-29c in NPC had a greatly difference through SPSS17.0single-factor analysis of variancetest,(P<0.05), but there was no significance between NPC before or afterchemotherapy.(2) By the Immune-Histochemical detention of NPC andnasopharyngeal mucosal chronic inflammation, we found SOCS-1wasnegative in NPC while there were25positive in nasopharyngeal mucosalchronic inflammation. We think maybe there was a relation between them.So, by the SPSS17.0Correlation analysis, we defined that miR-19b wasnegative with SOCS-1, and the correlation coefficient was0.573. AndCOL1A1was negative in the both. So, by the SPSS17.0Correlationanalysis, we find there was no relation with them.Conclusions（:1）Seum miR-19b and miR-29c expressed higher in NPCcompared with non-tumor person. So, they may be one of the potentialbiomarkers for clinical diagnosis of NPC.（2）The negative expression ofSOCS-1in NPC compared with non-tumor person declared that miR-19bmaybe inhibit the translation by matching with the target mRNA ofSOCS-1, and then affect cell proliferation and apoptosis by STAT.