The Research On Drug Resistance In H. Pylori Strains Clinical Isolated From Different Nationalities In Xin Jiang

Object: To investigate the antimicrobial agents resistant status of Helicobacter pylori(H.pylori)to metronidazole, clarithromycin, amoxycillin, levofloxacin, erythromycin, tetracycline andforoxone, which isolated from patients suffered with upper gastrointestinal diseases in Xinjiangarea. What’s more, in order to provide the evidences on treatment scheme of H.pylorieradication, to guide clinical drug use, to arrive at higher H.pylori eradication rate bycomparatively analysing the drug resistance to seven antibiotics above in H. pylori strains fromHan, Uygur, Kazak in Xin Jiang.Methods:1. To collect gastric mucosa specimens from Han, Uygur, Kazak patients in XinJiang, whichundergoing gastroscopy because of gastrointestinal symptoms and14C breath test being positive.2. Under microaerophilic condition, to isolate and culture H. pylori90strains, of which Han,Uygur, Kazak all were30strains. To identify by Gram method staining, Smear, Fast Urease Test,Oxidase test and Catalase test.3. To detect metronidazole, clarithromycin, amoxycillin, levofloxacin, erythromycin, tetracyclineand foroxone resistence in H.pylori By E-test (Krby-Bauer was sensitive to foroxone in H. pylori).4. To analyse resistence of H.pylori to seven antibiotics above from patients with differentnantionality,sexuality, age and disease.Results：1. The metronidazole, clarithromycin, amoxycillin, levofloxacin, tetracycline,erythromycin andforoxone resistence rate in90strains H.pylori in XinJiang were60.00%,25.56%,2.22%,37.78%,7.14%,26.67%,4.44%.2.27of H.pylori strains were all sensitive to7antibiotics, the ratio at30.00%.22strains weredrug-fast to one of all7antibiotics, at24.44%.22strains were drug-fast to2of all7antibiotics, at24.44%.15strains were drug-fast to3of all7antibiotics, at16.67%.3strains were drug-fast to4of all7antibiotics, at3.33%. Only one strains was drug-fast to5antibiotics, at1.11%. Thoseresistent to metronidazole+clarithromycin/erythromycin+Levofloxacin was the top, at13strains.Followed by those resistent to metronidazole+Levofloxacin, at10strains, and those resistent tometronidazole+clarithromycin/erythromycin, at7strains.3.The resistent rates in H.pylori to7antibiotics above from patients with different nantionality,sexuality, age and disease were not statistical difference（P>0.05）.Conclusions：1. The H.pylori resistant rate to metronidazole was the top in XinJiang, followed by Levofloxacin,Clarithromycin, Erythromycin, so all above four antibiotics were not been used to eradicateH.pylori as first-line drugs. The H.pylori resistant rates to amoxycillin, foroxone and tetracyclinewere lower, so they could be used to eradicate H.pylori as the main antibiotics. There were already multiple resistant H.pylori strains in XinJiang.2. The H.pylori resistant rate to seven antibiotics above had nothing to do with patients’nantionality,sexuality, age and disease.