Genotyping And Drug Resistance Of Mycobacterium Tuberculosis In Aksu,Xinjiang

Objective:In this project,we collected 186 Uyghur tuberculosis clinical isolates from Aksu,Xinjiang as the research object.Using PCR technology and agarose gel electrophoresis technology to conduct MIRU-VNTR genotyping of Mycobacterium tuberculosis is the research content,to explore the related research of genotyping and drug resistance of Mycobacterium tuberculosis in Aksu,Xinjiang.The successful completion of this topic can be preliminary To understand the genotype of Mycobacterium tuberculosis clinical isolates in Aksu,Xinjiang,the correlation between Beijing genotype and drug resistance,the risk factors that affect the production and epidemic of clustering and multi-drug resistance,and provide for the prevention and control of tuberculosis in the region Value information.Methods: 1.Collect 186 strains of tuberculosis strains from 1 city in Aksu 8 counties and corresponding case information 2.MIRU-VNTR genotyping of Mycobacterium tuberculosis and calculate the recent transmission rate according to clusters.According to the results of drug susceptibility tests,understand the drug resistance of Mycobacterium tuberculosis in this area.According to the RD105 deletion gene detection method,the strains are divided into Beijing and non-Beijing strains to understand whether there is a correlation between Beijing type and drug resistance and clustering.3.Multi-factor logistic regression analysis was used to understand the risk factors affecting clustering,multi-drug resistance and epidemic respectively.Results: 1.The drug sensitivity test showed that the total drug resistance rate in this area was34.9%(65/186),the initial drug resistance rate was 17.5%(20/114),the initial drug resistance rate was 15.8%(18/114),and the drug resistance The drug rate was 62.5%(45/72),the rate of re-treatment of multi-drug resistance was 47.2%(34/72),the results showed that the rate of initial drug resistance was significantly lower than that of re-treatment,and the rate of initial treatment of multi-drug resistance was significantly lower than that of The rate of treatment and multi-drug resistance was statistically significant(P<0.05);2.Beijing-type gene detection analysis showed that 101 strains of Beijing type and 85 strains of non-Beijing type.Beijing-based drug resistance rate is 38.6%(39/101),multi-drug resistance rate is 31.7%(32/101),non-Beijing drug resistance rate is 30.6%(26/85),multi-drug resistance rate is23.5%(20/85),The difference was not statistically significant(P>0.05).3.MIRU-VNTR genotyping showed a total of 156 genotypes,including 129(69.4%)unique genotypes,withmore than 27 clusters,2-3 plants per cluster,a total of 57(30.6%)plants,with a clustering rate of 16.1 %.There is no significant difference between the cluster type and the unique type in terms of gender,smoking history,COPD,malnutrition history,education level,and family annual income(P>0.05).Differences in MDR and treatment history are statistically significant and are independent risk factors for clustering of patients(recently spread).Chronic obstructive pulmonary disease,retreatment,treatment interruption or failure,and pulmonary cavity are independent risk factors for MDR-TB.Conclusion:The clinical isolates of Mycobacterium tuberculosis in Aksu area showed obvious gene polymorphism;multi drug resistance and retreatment were the important risk factors for clustering,that is,short-term transmission;chronic obstructive pulmonary disease,retreatment,treatment interruption or failure,pulmonary cavity were the independent risk factors for MDR-TB.