The Correlation Study Between C1GALT1Gene And ST6GALNAC2Gene With Immunoglobulin A Nephropathy In Xinjiang Uyghur People

Objective：To explore the relationship among single nucleotide polymorphisms of C1GALT1gene (rs9639031，rs5882115and rs1047763)、ST6GALNAC2gene(-988A>G、rs1867561)and genetic susceptibility of immunoglobulin A nephropathy in Xinjiang Uyghur people.Thisprovided reference basis for genetic research of immunoglobulin A nephropathyMethods: Random selecting Xinjiang Uyghur population IgA nephropathy patients90cases,compared with90normal control, extracted venous blood, and picked up DNA. For theextracting DNA, proceeded polymerase chain reaction (PCR), and PCR products were useddirect sequencing analysis. Directly using method of counting to calculate of alleles andgene frequencies type. Type of gene proceeded Hardy-Weinberg balance estimates, otherstatistical analysis completed with SPSS17.0software, comparing of genotype frequenciesand allele frequencies made use of the chi-square test(χ2test).Results：(1) In C1GALT1gene rs5882115, frequency distribution of every genotype andallele both had significant difference (P=0.014for genotype,P=0.005for alleles) in IgANgroup and control group. in IgAN group DD、DI and II genotype and D, I allele frequencieswere65.6%、32.2%、2.2%、81.7%and18.3%respectively, and in control group theyrespectively were84.4%、14.4%、1.1%、91.7%and8.3%. I alleles more significantlydifference than the D alleles (P=0.005), so it may was IgAN risk factors (OR=2.469). DIgenotype more significantly difference than the DD genotype (P=0.004), so it may increasedthe risk of IgAN (OR=2.874).(2) C1GALT1gene rs9639031, in IgAN group CC、CT and TTgenotype and C, T allele frequencies were40.0%、42.2%、17.8%、61.1%and38.9%respectively, and in control group they respectively were32.2%、46.7%、21.1%、55.6%and44.4%respectively, the difference was not statistically significant（P>0.05）；(3) C1GALT1gene rs1047763, in IgAN group AA、AG and GG genotype and A, G allele frequencies were21.1%、47.8%、31.1%、45.0%and55.0%respectively, and in control group theyrespectively were17.8%、40.0%、42.2%、37.8%and62.2%respectively, the difference wasnot statistically significant（P>0.05）；(4) ST6GALNAC2gene-988A>G, in IgAN group AA、AG and GG genotype and A, G allele frequencies were27.8%、46.7%、25.6%、51.1%and48.9%respectively, and in control group they respectively were20.0%、42.2%、37.8%、41.1%and58.9%respectively, the difference was not statistically significan（tP>0.05）；(5) InST6GALNAC2gene rs1867561, frequency distribution of every genotype and allele bothhad significant difference (P=0.006for genotype,P=0.001for alleles) in IgAN groupCC、CG and GG genotype and C、G allele frequencies were30.0%、52.2%、17.8%、56.1%and43.9%respectively, and in control group they respectively were52.2%、40.0%、7.8%、72.2%and27.8%. G alleles more significantly difference than the G alleles (P=0.001), so it may wasIgAN risk factors (OR=2.034). In IgAN patients and controls CG and GG genotype had significant difference than the CC genotype (P=0.012, P=0.005), so it may increased the riskof IgAN (OR=2.273, OR=3.979).Conclusion:(1) C1GALT1gene rs5882115polymorphisms has some relation with risk of IgAnephropathy in Xinjiang Uyghur people, maybe increased genetic susceptibility of IgAnephropathy in Xinjiang Uyghur people. I alleles and DI genotype possibly were risk factorsof pathogeny.(2) ST6GALNAC2gene rs1867561polymorphisms has some relation with risk of IgAnephropathy in Xinjiang Uyghur people, maybe increased genetic susceptibility of IgAnephropathy in Xinjiang Uyghur people. G alleles、CG genotype and GG genotype possiblywere risk factors of pathogeny.(3) In the study population, we had not yet find that there was relationship between singlenucleotide polymorphisms of rs9639031、rs1047743and-988A>G sites genetic susceptibilityof IgA nephropathy in Xinjiang Uyghur people.