The Differentially Expressed Genes Screen In Asthmatic Rats And Conduct The Interferential Research Of Chaipu Decoction

ObjectiveTo investigate the abnormally expressed genes between asthmatic rats andChaiPu decoction intervention ones during different times. To investigate themechanism of asthma and explore target genes of ChaiPu decoction.MethodsForty-two SD rats，weight(150-200）g, were randomly divided into a controlgroup,a asthma model group and a chaipu decoction-treated group. Asthma rat modelwas established by sensitization and stimulation with albumin(OVA) conjuncted withAl(OH)3. Executing asthma rats after exciting asthma2weeks,4weeks and8weeksrespectively,collecting bronchoalveolar lavage fluid （BALF） to count theinflammation cells and eosinophil cells,obtaining lung tissue for HE staining andpathology image analysis,extracting lung tissue RNA and then used Gene chip toscreen and real time-PCR to prove it.Using gene expression times numerical2.0and-2.0for threshold to determine the differential expression genes，then GO andpathway analysis were used to analyse the differential expression genes on functionand classification.Results1. The inflammatory cells in BALF were counted and classified in each groupThe number of inflammatory cells and eosinophils were significantly lower thanasthma model group, the difference was significant (P<0.01); As time prolonged, thecell viability behaved the same way in asthma model group and ChaiPu decoctionintervention group. There was a significant difference between each groups (P<0.01),The munber of inflammatory cells and eosinophils number in ChaiPu decoction in theintervention group is lower than that of asthma model group, with significant differe- nce (P <0.01).2. The changes in airway remodeling of asthmatic ratsStimulated rats after2weeks of asthma began to appear a large number of infl-ammatory cell infiltration around the bronchial wall, airway smooth muscle thicken-ing, goblet cell hyperplasia with mucus hypersecretion, excessive collagen deposition,basement membrane thickening; The airway smooth muscle and airway wall is thickerin four-week asthmatic group than two–week asthmatic group, the difference wassignificant (P <0.01); The airway smooth muscle is clearly thicker and the lumen ismore narrow in eight-week asthmatic group compared with than four–week asthmaticgroup, the difference was significant (P <0.01).3. Each groups rats gene chip screening and real-time PCR verificationAnd13two-fold DEGs were screend from the24358gene expression spectrum.These differently expressed genes are involved in defense responses, immune respo-nse, cell apoptosis, neuromodulation of asthma. New genes such as Mal、TPD52L1participate in the procession of asthma,which was proved by real-time PCR and genechip technology.Conclusion1.The abnormal expression of Mal、TPD52L1in lung tissue affect the progression ofasthma.2. ChaiPu decoction relieve asthma airway inflammation and airway remodeling proc-ess by up-regulating expression of Mal and down-regulating expression of TPD52L1.