Rangeability Of Intracellular Calcium Ion Concentration In Prostate Smooth Musclc Cells Of SD Rats With Chronic Abacterial Prostatitis In High Potassium Solution

Objective To establish a model of chronic abacterial prostatitis （CAP） of SD rats，Toinvestigate rangeability and clinical significance of intracellular calcium ionconcentration in prostate smooth muscle cells of SD rats with chronic abacterialprostatitis in high potassium solution.Methods We stochastically divided SD rats into experiment group and controlgroup（Each group number20）,established CP model by castration and estradiolinjection，and cultured and purified the PSMC in vitro. Laser confocal scanningmicroscope was used after the cells were incubated with Quest Fluo-8TMinside theincubator. Then the cells were dealed with high potassium solution, and Rangeability offluorescence intensity was dynamically observed.Results The pathologic specimens of the experiment group showed typical pathologiccharacteristics of chronic prostatitis under light microscope, the control group did notsee inflammation performance. Using immunocytochemistry method confirmed that theexperiment group and the control group were prostate smooth muscle cells. TheRangeability of fluorescence intensity of [Ca²⁺]i in the experiment group and controlgroup in the high potassium solution was27.86±9.88and7.61±4.31，there werestatistically significant differences between the two groups（P<0.01）.Conclusion The castration and estradiol injection method can be successfullyestablished CP model.The rangeability of intracellular calcium ion concentration of PSMCs in the CAP models in high potassium solution was greater than that of thecontrols. High potassium solution cause the flow across the membrane of extracellularcalcium ion, and so that intracellular calcium ion concentration increases.This affectsphysical function of prostate and its cells, which may be involed in the pathogenesis ofCP.