Quantitative Expression Of Voltage Dependent Calcium Channels In Rat Prostate Smooth Muscle Cells With Chronic Nonbacterial Prostatitis

Objective: To establish a rat model of chronic abacterial prostatitis(CAP) and quantitative measure the expression of voltage-dependent calcium channels(VDCC) of prostate smooth muscle cells (PSMCs),investigate the difference between model group and control group, to discuss the role of VDCC in CAP.Methods: We established an estrogen-induced (ih 0.25mg/kg,qd,for 30d) CAP model of rat.Dissected prostate in aseptic condition. Part of tissue used for HE staining,others for cell culture. PSMCs were cultured in vitro and purified.Immunocytochemistry staining were used to identify the expression of specific smooth muscle antibody. Trizol was used on extract total RNA of PSMCs. Via reverse transcription reaction,Double standard curve SYBR Green I real time PCR was used on measure mRNA expression ofα1 subunit in calcium channel subtypes.Results: A large number of spindle-shaped and polygonal cells were cultured in vitro,the cells grew as a whirlpool when became confluent,accorded with the growth characteristic of smooth muscle,and immunocytochemistry staining result confirmed the PSMCs with positive expression ofα-SMA.In CAP group,prostate tissue was infiltrated by lymphocytes and plasma cells , consistent with pathological characteristics of chronic inflammation. The control group without prostatitis.The expressions of L,T,P/Q-type calcium channel were found both in CAP group and control group, and the expression of L-type calcium channel in CAP group was increased in contrast to control group,respectively 0.048±0.024 and 0.031±0.015( t=2.846 P=0.007), but there were no statistical significance in T-and P/Q-type calcium channels between them.Conclusions: The results indicated that the number of L-type calcium channel of PSMCs increased in CAP.It might cause the Ca2+ inflow and induce smooth muscle tension in prostate ,blade outlet and pelvis floor Otherwise,L-type calcium channel play an important role in sustaining chronic pain,So we speculated that L-type calcium channel involved in the mechanism of CAP.