| Objective: To complement external phosphoric acid creatine (CP), whether canimprove the body CP reserves, whether can improve the anaerobic exercise of aging,reduce the role of free radical damage is the purpose of this study. Scientific training toimprove performance, protect the sport athletes don’t hurt, increased life expectancyathletes play a crucial role. The human body motion of direct energy is adenosinetriphosphate (ATP). Muscle contraction is to use the ATP decomposition release energy,for muscle contraction overcome resistance work, so as to realize the transformation ofchemical energy to the mechanical energy. ATP in muscle in content is very limited, ifonly in the most power output can keep2seconds or so, then the ATP and phosphoricacid creatine (CP) of the original system composed of phosphoric acid (Phosphagenstem) can, duration of7.5seconds (or so). When the original system energy shortage ofphosphoric acid, the human body to be utomatic enable lactic acid can system, andfinally Aerobic oxidation system (Aerobic oxidation system); To provide energy forhuman body movement. The original system is all phosphoric acid high power outputmaterial base of the sport, several seconds to play within the maximum energy output,so how to the increase in phosphoric acid creatine reserves to powerful explosive force,speed of anaerobic exercise program is very important. Directly added at home andabroad about the research mainly creatine phosphate in clinical, mostly for heart surgery,joined the cardiac arrest in the fluid of myocardial protection, or prevent the myocardialischemia state metabolic abnormalities. In sports training and competition andphosphoric acid creatine application less, but from the90s, a large number of studiesshowing that, creatine for high strength of sprint’s running, swimming, bicycles andsome intermittent, many times the power of the repeated outbreaks of sport ability ofproject was improved effect, and do not belong to the international Olympic committee(ioc) listed in the illicit drug scope, short-term use not seen obvious side effects, and therefore become a domestic and international sports and science of attention a hot andthe number of application in sports training and competition, and have achieved goodeffect. But in fact about60%-65%of the creatine and phosphoric acid, and thecombination of other one-third of creatine with free creatine exists in the form of. Sodirectly added phosphoric acid creatine has more practical meaning. The experiments tokunming adult mice as the research object, the mice take exogenous added phosphoricacid creatine mode, through to the mice ATP, creatine kinase (CK), lactic acid (LD),calcium Ca2+indices such as testing, more supplement creatine phosphoric acid toimprove the ability of anaerobic exercise the function of support.Methods:Kunming mice health only50, months of February, weight and±3g.Temperature25±2o C, relative humidity50-5%, the mice points raised cage, the onlytwo cage13, the only two cage12, natural light, free to eat, drink, mice fed day2adaptability swimming training, for mice sport ability is not strong, weight appears afterdeath. Stop swimming weight training, in accordance with the above conditionscontinue to feed.12days all starting to daily swimming training in mice. Swimmingtraining, with towel dry and blower hair dry rat. To the end all round, the mice weight40±3g. Random into four groups, group A (n=12): don’t give medicine exhaustiongroup; B group (n=12): give medicine exhaustion group; C group (n=10): don’t givemedicine8min exercise group; D group (n=10): give medicine8min exercise group.Group B group D intraperitoneal injection of phosphoric acid creatine (1000mg/kgweight) A group of group C intraperitoneal injection of saline. Group D group C weightloading swimming (weight6%weight) eight minutes after the ends immediatelyremoved to be put to death, quick take blood, centrifugal eyes; Take quadriceps grouphomogenate. A group of group B weight loading swimming (weight6%weight) toexhaustion immediately after the ends executed, and rapid eye socket, centrifugal takesome blood; Take quadriceps group homogenate. Four out of the group quadricepsgroup homogenate centrifugal, detection after quadriceps group of ATP, creatine kinase(CK), lactic acid (LD), calcium Ca2+index.Results:1, exhaustion movement the change of time: give medicine swimming group(group B) average significantly prolongs the time of swimming in mice, and swimgroup (group A), compared to an average23.20%extend athletic time.2, the biochemical changes:ATP content: give medicine8min exercise group (group D) muscle tissue ATP average of3.64+/-1.17(umol/gprot) and not give medicine8min exercise group(group C), don’t give medicine exhaustion (group A), drug exhaustion (group B)compared with significantly increased (P﹤0.01); Don’t give medicine8min exercisegroup (group C) and not give medicine exhaustion (group A), drug exhaustion (group B)compared with increased, but (P>0.05) were not significant.Creatine kinase (CK) content: give medicine8min exercise group (group D)muscle tissue CK average of2.69+/-1.04(u/mgprot) and not give medicine8minexercise group (group C), don’t give medicine exhaustion (group A), drug exhaustion(group B) compared to low (P﹤0.05); Don’t give medicine8min exercise group(group C) and not give medicine exhaustion (group A), drug exhaustion (group B)compared with reduced, but (P>0.05) were not significant.Lactic acid (LD) content: don’t give medicine exhaustion control group (group A)mice in the musculature of the LD content and injection phosphoric acid creatineswimming exhaustion group (group B) compared with no significant difference (P>0.05), but the drug was higher than to the medicine and8min exercise group (D group,the group C). Don’t give medicine8min exercise group (group C) and give medicine8min exercise group (group D) mice in skeletal muscle in LD difference, but not obvious.Calcium Ca2+content: don’t give medicine exhaustion in control group (group A)the musculature of the mice in calcium and injection phosphoric acid creatineswimming exhaustion group (group B) compared with no significant difference (P>0.05), but the drug was higher than to the medicine and8min exercise group (D group,the group C). Don’t give medicine swimming8min exercise group (group C) and toadvance in phosphoric acid creatine (D group) mice swimming after8min, mice2+content in skeletal muscle Ca, there are differences. Group C above group D.Conclusion: exogenous phosphoric acid creatine can obviously increase sportability of mice, reduce the free radicals formed, reduce the oxidative stress free radicalson the role, maintain stable cell membrane, has the function of protection of cells, aswell as reduce lactic acid produce, thus delay the generation of exercise-induced fatigue. |
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