Breviscapine Joint PNS On Acute Cerebral Ischemia Models Of Effects Of TNOS And INOS

Objective and significanceIn recent years, in our country, cerebrovascular disease have been riseing for the frist fatal disabling conditions. Annual death toll is about150million, among them with ischemic cerebrovascular disease incidence of all stoke highest70%, new method is needed for the treatment of ischemic cerebrovascular disease in clinical therapy.Cerebral infarction of ischemic cerebrovascular disease, refer to the brain blood circulation barrier, cause blood supply or lack interruption, make its supply of brain ischemia and hypoxia and dead softened. In addition, studies show that NO and NOS in ischemic brain injury has an important role. NOS as the key enzymes for the synthetic of NO. Lots of researchs show that TNOS expression increase in cerebral ischemia, catalytic synthesis of excess NO, thus participate in ischemic brain injury pathophysiological events. Participate in nerve darmage is generally considered the main axle by guide to living iNOS type NOS catalysis. The iNOS is pathological status enzyme, expression of iNOS in a large number when cerebral ischemia participate in delayed neurotoxivity, mainly activated glial cells, neutrophils and macrophages produce, of brain ischemia, the latter an important factor in aggravating brain damage. Thus, inhibition of TNOS content and activity of iNOS expression is considered one of the cerebral infarction treatment way. There is long history for the traditional Chinese medicine panax and breviscapine that treatment the cerebral infarction, curative effect, with the long-term efficacy stability, adverse reaction, side effects and so rare, But the current combination of two medicines have not been reported at home and abroad, the study on the mechanism is rare. This research use Panax combine Breviscapine intervention cerebral infarction in the rat model, by observing the Panax combine Breviscapine in rats with cerebral infarction TNOS content and iNOS activity and mechanism of change, Explore the impact of cerebral infarction on serum TNOS content and charge of the activity of iNOS, Reveal the mechanism of treatment of cerebral infarction, the theoretical basis for clinical application.Methods50adult male SD rats were randomly divided into five groups:sham operation group, model group, Breviscapine group, PNS group and Traditional Chinese Combination group, each group of10SD rats, using suture model of middle cerebral artery occlusion.2hours after administration, once daily qd for3days. After each group were taken at6h,24h,72h venous blood,1hour after the last blood brains were decapitated brain. TNOS content and iNOS vitality changes were measured by chemical colorimetric of serum. Neurological deficit score using BedersonlO scores law standards, infarct area was calculated based on percentage. HE staining observed under light microscopy ischemic cerebral infarction histopathologic changes.Results1. Panax combine Breviscapine can effectively reduce the symptoms of animal models of cerebral ischemia.2. Panax combine Breviscapine can significantly reduce the neurological deficit of cerebral ischemic72h animals (P=0.001), compared with the single drug group, the diffence was statistically significant (.P<0.001).3. Panax combine Breviscapine can significantly reduce cerebral infarction rats serum vigor of iNOS and TNOS content (P<0.005), compared with the single drug group were significantly different (P<0.005).ConclusionPanax combine Breviscapine by reducing cerebral infarction rats serum TNOS content and iNOS vitality, increase blood flow to prevent cerebral ischemic injury, achieve the purpose of treatment of cerebral infarction. It may be one of the mechanisms of theraping of cerebral infarction.