The Protective Effect And Mechanism Of Breviscapine On Type2Diabetes Mellitus Rats With Acute Myocardial Ischemia

[Objective]â‘ To study the change of fasting blood glucose (FBG)/creatinekinase isoenzume-MB (CK-MB)/Cardiac Troponin I (cTn-I)/insulin (INS)/total cholesterol (TC)/triglycerides (TG)/high-density lipoproteinc holesterol (HDL-C)/low-density lipoprotein cholesterol (LDL-C) and the expression of adiponectin (APN), and discuss the mechanism of the type2diabetes mellitus rats with acute myocardial ischemia.â‘¡To study the probable mechanism of breviscapine on prevention and treatment of the type2diabetes mellitus rats with acute myocardial ischemia.[Methods]65healthy and male SD rats were adaptively fed for7days, and theirFBG after12h were measured. Choose62rats with normal FBG, which randomlydivided into the normal control group (NG) with12rats (normal feed), the high-fatand high-carbohydrate group with50rats (feed on high-fat and high-carbohydratediet). After28days NG were injected with equal volume citric acid buffer, high-fatand high-carbohydrate group were injected with40mg/kg streptozotocin intointraperitoneal. After5days, the rats with the fasting blood glucose greater than16.7mmol/L are the type2diabetes mellitus rats. The type2diabetes mellitus ratswere randomly divided into the model group (MG), the low dose group (LG), and thehigh dose group (HG), with12rats in each group. Breviscapine was given at the dailydose of100mg/kg and200mg/kg by injection in the LG and HG group for14d.Meanwhile, Equal volume of physiological saline was given to rats in the NG andMG by injection for14d. Myocardial ischemia injured models were established by subcutaneous injection of ISO (6mg/kg, continuous3d) except NG. After the lastadministration, all rats anaesthetized by injecting chloral hydrate were connected tothe electrocardiogram, Readings were recorded separately after5minutes,10minutes,30minutes and60minutes. The standard of myocardial ischemia was the offset of Jpoint in ECG. At the end of the experiment, all rats were executed and blood samples,pancreas and heart tissue were collected. Serum were collected by centrifugation, INSand such items were calculated as the insulin resistance index (HOMA-IR).TC/TG/HDL-C and LDL-C were measured in order to detect the degree of glucoseand lipid metabolism in the body. CK-MB and cTn-I are measured to detect thedegree of myocardial injury. The structural changes of pancreatic and heart tissuesstained with hematoxylin and feosin (HE) were observed under a microscope. Theexpressions and distribution of APN in the heart tissues are analyzed by means ofimmunohistochemistry. The serum level of APN were measured by ELISA.[Results]1. Serum index detection: Compared with NG, FBG/CK-MB/cTn-I/INS/TC/TG/LDL-C in MG was significantly higer (P<0.05), APN was significantly decreased (P<0.05), and there was no significant alteration of HDL-C. In thedrug intervention group, compared with MG, FBG/CK-MB/cTn-I/INS/TC/TG/LDL-C was significantly decreased (P<0.05), APN was significantly higher (P<0.05), and there was no significant alteration of HDL-C. compared with LG, FBG/CK-MB/cTn-I/INS/TC/TG/LDL-C was significantly decreased (P<0.05), APNwas significantly higher (P<0.05), HDL-C lower instead (P<0.05).2. Results in HE staining:â‘ The structural changes of pancreatic: The normalpancreatic tissue structure was a complete form which was round or class round andhave scattered between cells, the cytoplasm was plenty, the number of cells were alsolarge. In MG, the number of islet was little, volumes density and numbers decreased,cytoplasm of dyeing becomes shallow, cell morphology was irregular, some nucleus pycnosis. HG and LG compared with MG were obviously improved: the number andcytoplasm of islet cell were increased, nucleus pycnosis was decrease, histologicastructure was a better form, especially in HG.â‘¡The structural changes of heart:The normal myocardial fibers was neat and had no inflammatory cells inmesenchymal. In MG: myocardial cells widely edema/fatty degeneration andNecrosis, amount of inflammatory cell infiltration, interstitial edema and hemorrhagewere observed. LG and HG compared with MG, myocardial cell degeneration andnecrosis were significantly decreased, espcially in HG.3. Immunohistochemistry results: APN was located in the cytoplasm, and the darkbrown particles seen here was expressesed being positive. The APN in myocardialcell was practically expressed. The expression of APN in MG was raised comparedwith NG (P<0.05). The level of APN in LG and HG was increased, more than that ofMG (P<0.05), And the expression of APN was more than that of LG (P<0.05).4. ELISA results: the APN level of MG decreased compared with NG (P<0.05); HGand LG were increased compared with MG (P<0.05); and HG was increasedcompared with LG (P<0.05).[Conclusions]â‘ In the type2diabetes mellitus rats with acute myocardial ischemia,the glucolipid metabolism/insulin resistance/pancreas and myocardium tissue wereserious injury, the expressions of APN in the myocardium was declined.â‘¡Breviscapine can play a role in protecting pancreas and myocardium throughadjusting energy metabolism/improving insulin resistance and hypoadiponectinemia.