The Relationship Between Metabolic Syndrome And Sex Hormones And Apolipoprotein E Gene Polymorphism In Elderly Population In Urban Community

Objective: The purpose of this study is to understand the prevalence rate of themetabolic syndrome in the elderly population of the urban community. To explorethe correlation of the metabolic syndrome and sex hormones in elderly. Analyze thecorrelation of the metabolic syndrome and apolipoprotein E gene polymorphism.Methods: Typical sampling method was adopted, selecting five communitieswith higher proportion of elderly population in the city of YinChuan and WuZhong.All met the inclusion and exclusion criteria face-to-face questioned and examined(including height, weight, waist circumference, hip circumference and bloodpressure).The blood samples were drawed to test the fasting blood glucose,triglycerides, cholesterol, high-density lipoprotein cholesterol, low-densitylipoprotein cholesterol and other indicators of detection, and test the serum totaltestosterone (TT), estradiol (E2), luteinizing hormone (LH) and follicle-stimulatinghormone (FSH), sex hormone binding globulin (SHBG),while the whole blood DNAwas extracted with a Promega DNA Kit, and use high resolution dissolving curvemethod for detecting the single nucleotide polymorphism of apolipoprotein E genein rs429835and rs7412loci, using SPSS16.0software for statistical analysis.Result:1.The survey of prevalence of MS in elderly population of urban community.1) The prevalence of the MS was43.3%, while the man was42.9%and thewomen was43.3%. no significant differences in prevalence rates of the male andfemale; Whereas ethnic-specific prevalence of the MS was43.9%in Han ethnic group and42.1%in Hui ethnic group, no significant differences in prevalence ratesof the Han and Hui ethnic group.2) No significant differences in prevalence of all age groups between the Hanand Hui ethnic group in elderly men, as well as in the elderly women. While nosignificant differences in standardized prevalence of all age groups between the Hanand Hui ethnic group in elderly men, and the elderly women was the same. Nosignificant differences in prevalence of all age groups between the male and female,as well as in the standardized prevalence.3) Whether in elderly men and women, the prevalence of high blood pressurewas highest, followed the fasting blood glucose.4) The prevalence of the MS in elderly wen and women does not rise withincreasing age. unconditional Logistic regression results show: salty diet, familyhistory of diabetes were the risk factor of elderly MS.2. The correlation of the sex hormones and MS in the urban elderly population.1) In elderly men, the body weight, waist circumference, hip circumference,BMI, systolic blood pressure, diastolic blood pressure of the MS group wassignificantly higher than control group, and no significant difference was observed inages between the two group. The FBG, TC, TG, LDL-C of apoB, uric acid, FINS,HOMA-IR of the MS group was significantly higher than control group, whileHDL-C, apoA1was significantly lower than the control group. No significantdifference in total bilirubin, albumin between the two group.2) In elderly men, The TT of the MS group was significantly lower than thecontrol group, and no significant difference in E2, E2/TT, FSH, LH, SHBG betweentwo groups. The age group of55to64: the TT of the MS group was significantlylower than the control group, and no significant difference in E2, E2/TT, FSH, LH,SHBG between the two groups. The age group of65to74: The TT of the MS group have decreased compared with the control group, and the E2have increased, but nostatistical significance, The E2/TT of the MS group was significantly lower than thecontrol group, and no significant difference in FSH, LH, SHBG between the twogroups. The age group of75and above: The TT of the MS group was significantlylower than the control group, and no significant difference in E2, E2/TT, FSH, LH,SHBG between the two groups. Conditional Logistic regression analysis showed thatTT reduce, LDL-C increased were the risk factors of the MS in elderly men.3) In elderly women, the body weight, waist circumference, hip circumference,BMI, systolic blood pressure, diastolic blood pressure of the MS group wassignificantly higher than the control group. There was no significant difference inage, age at menopause, menopause time, number of pregnancies, production timesbetween the two groups. The FBG, TC, TG, LDL-C, apolipoprotein B, uric acid,FINS, HOMA-IR of the MS group was significantly higher than the control group,while the HDL-C, apolipoprotein A1of the MS group was significantly lower thanthe control group. No significant difference in total bilirubin and albumin betweentwo groups.4) In elderly women, the E2, FSH, LH of the MS group was significantly lowerthan the control group. There was no significant difference in TT, E2/TT, SHBGbetween the two groups. The group of menopause time≤10years: the E2of the MSgroup was significantly lower than the control group, and no significant difference inTT, E2/TT, SHBG between the two groups. The group of menopause time11-20years: the E2, FSH, LH of the MS group was significantly lower than the controlgroup. There was no significant difference in TT, E2/TT, SHBG between the twogroups. The group of menopause time≥21years: no significant difference in TT, E2,E2/TT, LH, FSH SHBG between the two groups. Condition Logistic regressionanalysis showed that the E2decreased and uric acid, apoB/A1increased were the risk factors of MS in the elderly women.3. The correlation of the apolipoprotein E gene polymorphism and the MS inthe elderly population.1) The genotype frequencies of the six genotypes E2/2, E2/3, E2/4, E3/3, E3/4,E4/E4were2.1%,13.2%,0.3%,77.4%,6.3%, and0.8%, respectively. The E2, E3,E4allele frequencies were8.8%,87.1%and4.1%, respectively. No significantdifference in genotype frequencies between the male and female, and the allelefrequencies is the same. no significant difference in whether carrying E2between themale and female, and whether carrying E4is the same. No significant difference inwhether carrying E2or E4between the Han and Hui ethnic group.2) There was no significant difference in genotype frequencies between the MSgroup and the control group, and no significant difference in allele frequenciesbetween two groups whether carrying E2or E4.3) The DL-C, apoA1level in carrying E2group was significantly higher thanthat without carrying, while the TC, LDL-C level in carrying E2group wassignificantly lower than that without carrying. There was no significant difference inFBG, SBP, DBP, BMI between two groups. The TC, LDL-C level in carrying E4wassignificantly higher than that do not carry, no significant difference in TG, HDL-C,apoA1of apoB, FBG, SBP, DBP, and BMI level between two groups.Conclusions: The prevalence of MS in elderly population that is more than55years old in the community of Ningxia was higher. Reduced TT and elecated LDL-Cmay be associated with MS in elderly men, while reduced E2,elecated uric acid andapoB/A1may be associated with MS in elderly women. There was no correlationbetween the APOE gene polymorphism and MS in elderly population.