Matrine With Cisplatin Inhibit The Proliferation Of Lung Cancer A549Cellsand Synergy

Objective:To study the different concentrations of matrine (matrine, MT) inhibitionof proliferation of non-small cell lung cancer A549cells, cell cycle and apoptosis, andsynergy with cisplatin combined for flavescensalkali new treatment ideas and theoreticalbasis for the application in the treatment of lung cancer.Method:Non-small cell lung cancer A549cells were cultured in vitro experimentstake the logarithmic growth phase cells, using methyl thiazolyl tetrazolium (MTT) assaydifferent concentrations of matrine group (30,60,120mg/L)cisplatin group(0.625ug/ml), matrine cisplatin treatment group. Role in lung adenocarcinoma A549cells24,48, and72hours after, respectively, to calculate the rate of cell growth inhibition.The impact of drugs on cell morphology was observed under an inverted microscope.Propidium iodide (PI) staining and flow cytometry to detect cell cycle. Matrine (30,60,120mg/L) group and matrine cisplatin combination group were detected. Observe themrole in lung adenocarcinoma A549cells48hours after cell cycle changes. Annexin V/PIdouble staining matrine (30,60,120mg/L) group and matrine combined with cisplatingroup to handle the A549cell apoptosis rate after48hours.Results:1. Matrine on A549cell proliferation inhibition and matrine inhibit the proliferationof lung adenocarcinoma A549cells gradually increased with the increase of time andconcentration of drug action, show time-dose dependence; matrine Heshun platinumgroup combined effects of lung adenocarcinoma A549, there is also inhibit theproliferation and the inhibition was significantly higher than single-drug treatment group,also was a time-a dose-dependent, especially in the intervention at48and72hours later,the inhibitory effect of the combined treatment group is more apparent. 2.Different concentrations (30,60,120mg/L) the Matrine group, after48hours oftreatment, with the increase of the concentration of the drug, compared with the negativecontrol group, the proportion of G1phase cells gradually increased the proportion ofS-phase cells gradually decreased the cisplatin treatment group Gl phase cells decreased,while the increase in the proportion of cells in S phase; matrine combined treatmentgroup, found that the proportion of cells in G1phase matrine treatment group comparedwith the same dose cisplatin than cisplatin treated high compared matrine treatmentgroup, while the proportion of cells in S phase, lower than the cisplatin treatment group,the matrine block the lung adenocarcinoma A549cells in G1phase, cisplatin block A549cells in the S phase of the two drugs joint, G1and S phase cells are blocked.3.Different concentrations (30,60,120mg/L) and cisplatin (0.625ug/ml) jointmatrine, matrine group (60mg/L) group acting on lung adenocarcinoma A549cells48hours after the negative the control group, the increase in the number of the matrinegroup of early and late apoptotic cells, and in a certain concentration range, theproportion of apoptotic cells increased with the increase of the concentration of the drug;the matrine cisplatin apoptosis cells is much higher than the proportion of matrinemonotherapy group (P <0.05).Conclusion: Matrine inhibit the proliferation of non-small cell lung cancer cell linesA549, within a certain range of concentrations, time and concentration-dependentproliferation inhibition than monotherapy group and cisplatin combined, illustrate twoafter drug combination has a synergistic effect; mechanism of inhibition of cellproliferation, and they block the cell cycle, induction of apoptosis-related.