Effect And Mechanisms Of Matrine On Proliferation In Human Ovarian Cancer Cell

ObjectiveTo study the effect and mechanisms of matrine on proliferation in human epithelial ovarian cancer OV2008 cell line in vitro.Methods3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay was used to examine the effects of matrine on proliferation in OV2008 cell at different concentrations (0 mg/mL,0.125 mg/mL,0.25 mg/mL,0.5 mg/mL,1.0 mg/mL,2.0 mg/mL,4. Omg/mL) and different time points (24h,.48h,72h). Flow cytometry (FCM) was used to analyze cell cycle, apoptosis rate and expression levels of Fas, Bcl-2 protein of OV2008 cell which were treated with matrine at different concentrations for 48h.ResultsThe results showed that matrine could obviously inhibit the proliferation of OV2008 cell, it had a significant difference compared with control group(P< 0.05 or P<0.01). The inhibition of matrine on OV2008 cell was in a time-and does-dependant manner. Cell cycles analysis indicated that the percentage of OV2008 cell in Go/G1phase was increased and in S phase was decreased while the concentration of matrine was increased, there was no obvious change in G2/M phase. The results also showed that matrine could induce apoptosis of OV2008 cell and the apoptosis rate was increased with the increased of matrine concentrations compared with control group(P＜0.01). Matrine also could increase the expression of Fas protein(P<0.05 or P<0.01) and reduced Bcl-2 protein expression(P<0.01).ConclusionsMatrine could obviously inhibit the proliferation of OV2008 cell and the inhibition of matrine on OV2008 cell was in a time-and does-dependent manner. The inhibition might be related with the arrest of cell cycles and the induction of apoptosis. The apoptosis of matrine inducted might be related with up-regulation of Fas protein and down-regulation of Bcl-2 protein.