Expressions Of Human Cytomegalovirus Infection In Craniopharyngiomas And The Significance

Human cytomegalovirus (HCMV) is a kind of beta herpesvirus. Recently somestudies showed that HCMV infection and its genes expression existed in multiple types ofhuman tumors, and which was associated with the degree of malignant tumors. Theseresults suggested that HCMV infection may play an important role in the formation andprogression of human multiple tumors, and HCMV infection may become a newpathogeny of human malignant tumors. Moreover, some researches indicated that HCMVgene productions involved in a variety of cell signaling pathway, such as inhibiting cellapoptosis, promoting cell proliferation, invasion, metastasis and angiogenesis and so on,as well as forming special immune escape mechanism to survive in the immune reactionsof body. To further investigate the etiology relations between HCMV infection and tumors,and its mechanism, may provide a new route for clinical prevention and cure of tumors. Craniopharyngioma is the most common congenital intracranial tumor in childrenand adolescents, about4%of intracranial tumors. The clinical manifestations ofcraniopharyngioma are usually complex and serious, very difficult to treat, resection isoften difficult to completely cut off, and the effect of radiotherapy and chemotherapy islimited, have a high recurrence rate and poor prognosis. The craniopharyngioma etiologyand pathogenesis is not fully clear, which is also an important factor in the lack ofeffective prevention and treatment methods. The correlation of HCMV infection and theformation and progression of craniopharyngioma had not been reported at home andabroad.PartⅠ Infection and expression of human cytomegalovirus and itssignificance in craniopharyngiomas89patients who were diagnosed with craniopharyngioma and had been operated inXijing Hospital from Jun2006to Dec2010were enrolled in this study, And10casesintracranial hypertension ostomy. Collected specimens during resection which werecompleted by experienced surgeon, fixed by formalin quickly and fully, paraffinembedded, cut4μm slide. Expressions of immediate early protein1-72(IE1-72) andphosphorylated glycoprotein65(pp65) antigens of HCMV were examined byimmunohistochemistry method. To reveal the expressions of human cytomegalovirus(HCMV) infection in craniopharyngioma tissues, and to investigate the etiological relationof HCMV infection with craniopharyngioma formation and progression.Result:HCMV IE1-72and pp65were stained in the cytoplasm and nucleus ofcraniopharyngioma, but unevenly distributed in the epithelial cells. The positive rates ofHCMV IE1-72and pp65expression were77.5%and84.3%respectively incraniopharyngiomas, but no expression of them in normal brain tissues; there weresignificant differences between both HCMV IE1-72and pp65antigen expression positiverates in craniopharyngiomas and normal brain tissues (P=0.000, P=0.000).The positive rates of IE1-72in adamantinomatous type and papillary type of craniopharyngiomas were83.6%and67.6%respectively, while pp65were89.1%and76.5%respectively; although both IE1-72and pp65expression positive rates inadamantinomatous type were little higher than them in papillary type, there was nosignificant difference between both (P=0.135, P=0.197). The positive rate of IE1-72inmen was81.6%, in women was72.5%; the the positive rate of pp65in men was81.6%, inwomen was87.5%; the positive rate of IE1-72and pp65were no significant differencebetween gender (χ2=0.595, P=0.440; χ2=0.215, P=0.643), or age (r=0.009, P=0.930;r=0.056, P=0.599).Conclusion:Both HCMV IE1-72and pp65were highly expressed in craniopharyngiomas, whileno expression of them in normal brain tissues, which implies HCMV infection andexpression may be associated with the formation and progression of craniopharyngioma.These may have a inspiration for prevention and treatment of craniopharyngioma.The positive rates of HCMV expression were no significance difference betweenadamantinomatous type and papillary type of craniopharyngiomas, but the positive rate ofadamantinomatous was slightly higher than papillary, these still prompted HCMVinfection may be related to the formation and progression of different histopathologicaltypes of craniopharyngioma. The rates were no significance difference between genderalso, which inconsistent epidemiological characteristics of HCMV infection, may berelated to the sample size was too small, or the expression of HCMV incraniopharyngiomas were related to gender. The pathogenic mechanism needs furtherstudy.PartⅡ Real-time PCR of human cytomegalovirus gene incraniopharyngioma tissuesCollect the right amount of fresh lesions from surgery for craniopharyngioma patientswho were diagnosed by pathology, and immediately placed frozen in liquid nitrogen, thenstored at－80℃refrigerator. The surgery was completed by an experienced surgeon.Exclude non-purpose specimens, such as cystic tissues and calcified tissue specimens, there were9cases in all. Quantitative detection of the HCMV nucleic acid incraniopharyngioma tissues, to further research the relationship of HCMV infected with theformation and progression of craniopharyngioma.Result:9cases organizations were all negative in HCMV gene quantitation, but1specimenhad positive trends, suggesting that HCMV nucleic acid may exist in the organization, butlittle content.Conclution:The level of HCMV nucleic acid in craniopharyngioma were very low, it can not beexcluded HCMV nucleic acid does not exist in the craniopharyngioma, and may beassociated with the size of experimental sample is small, experimental method is notoptimal, or the details to grasp is not good. Which may be related to the distibution ofHCMV infection in the body of the cell type or function, such as glands of body fluids.