Research On Evaluation Of Efficiency Of Stalks Degraded By White Rot Fungi With Cncps

Crop stalk provides for ruminants as abundant forage resources, but the majority of crop straw is difficult to be digested, resulting on the feeding value of limitation. Therefore, how to get a process of degrading to improve value of the nutrition, has been a research hotspot. The utilization methods of feed processing of straw include physical method, chemical method and microbiological method. In recent years, it has had some achievements on straw degrading by white rot fungi as a method of microbial treatment at home and abroad.There are many experiments used to evaluate the degraded stalk by in vivo methods and in vitro methods. However, no research focuses on the variation of specific components of two crucial matters in the stalk: protein and carbohydrate.Degradation by white rot fungi is one of the effective ways to improve the digestibility and provide ruminant with more available feed in future. In this study, four kinds of agricultural residues will be degraded by Pleurotus ostreatus, a representative species of white rot fungi, for different days (0days,7days,14days,21days and28days). Then it determined the enzyme activity of Pleurotus ostreatus in different days and in vitro gas production. The purpose of this study was to evaluate the samples bio-processed by the fungi with method named CNCPS (Cornell Net Carbohydrate and Protein System), on the basis of determining activities of three crucial enzymes in degrading process and measuring in vitro gas production of these samples. The study suggested that after a period of degradation, samples of four agricultural residues degraded by enzymes not only varied in CP, cellulose, hemicellulose and lignin, but also changed in certain fractions of protein and carbohydrate (all of PB2(P<0.01) except paddy straw, all of CC(P<0.01), etc.) respectively, which is important for rumen digestion. By determining the nutrients with CNCPS, it is helpful to understand fermentation of feed degraded by white rot fungi in rumen and to build a relationship between two stages of degradation: in vitro and in vivo.