Research On Bagasse Decomposed By Pleurotus Ostreatus For Fodder

Bagasse is one of important agricultural residues in southern of china, also is a potential fodder resource. Bagasse contains a lot of celluloses, hemicelluloses and lignins, a small amount of proteins, starches and soluble sugars. Bagasse has a high lignification. Bagasse decomposed by pleurotus ostreatus can be used as fodder, for the pleurotus ostreatus mycelium can secrete cellulase, hemicellulase and ligninase, then lignocellulose of bagasse is decomposed to small moleculars, which can be used as energy of pleurotus ostreatus or converted to digestible nutrients of livestock. On the one hand, a large number of pleurotus ostreatus myceliums can provide a lot of bacterial proteins.In order to improve the bagasse medium of pleurotus ostreatus, carbon source and nitrogen source were added into it. By analyzing nutrient composition, lignocellulose content and in vitro gas production parameters of bagasse decomposed by pleurotus ostreatus in different fermentation stages, we can evaluate nutritional value of bagasse decomposed by pleurotus ostreatus and select the best fermentation stage for bagasse as fodder. Detailed experimental results are as follows:(1) When bagasse medium was added by 10% rice bran and 4% soybean meal, it can meet the basic requirement of pleurotus ostreatus growth. After that, added 3% corn flour can enhance mushroom fiber enzyme activity, improve the pleurotus ostreatus growth and shorten the processing time.(2)After bagasse was decomposed by pleurotus ostreatus, the CP percentage of bagasse was increased, CF percentage of bagasse was decreased, lignocellulose of bagasse was degraded and the nutritional value of bagasse was improved as a result. There were three different fermentation stages:When bagasse medium has fully covered by mycelium of pleurotus ostreatus,formed pleurotus ostreatus bud and fruiting body of pleurotus ostreatus. The CP percentage of bagasse decomposed by pleurotus ostreatus was 228%,204% and 200% of bagasse in three different fermentation stages respectively; The CF percentage of bagasse decomposed by pleurotus ostreatus was 89%,86%and 83% of bagasse in three different fermentation stages respectively; Lignin degradation rate of bagasse decomposed by pleurotus ostreatus was 1.00%, 2.28% and 14.5% in three different fermentation stages respectively; Fiber degradation rate of bagasse decomposed by pleurotus ostreatus was 39.49%, 52.37% and 58.84% in three different fermentation stages respectively; Cellulose degradation rate of bagasse decomposed by pleurotus ostreatus was 28.08%,40.97% and 42.28% in three different fermentation stages respectively.(3) After bagasse was decomposed by pleurotus ostreatus, the mount of gas producted in vitro, IVOMD, ME and DE of bagasse were declined. With processing time extending, the mount of gas producted in vitro, IVOMD, ME and DE of bagasse were on the decline. NDF and ADF digestibility increased significantly at the early stage of the digestion, but the growth tendency was weak at the late stage of the digestion. When bagasse medium had formed fruiting body of pleurotus ostreatus, NDF and ADF digestibility of bagasse decomposed by pleurotus ostreatus were 16.56 and 18.56 times of bagasse respectively.(4) Considering the nutrients of the bagasse in different fermentation stages, when bagasse medium has fully been covered by mycelium of pleurotus ostreatus or bagasse medium has formed pleurotus ostreatus bud, bagasse could be used as fodder, which was better than the bagasse medium formed fruiting body of pleurotus ostreatus.