| In this study, the material is the three generation transgenic wheat with maize C4-pepc gene. We determined the net photosynthetic rate and PEPC enzyme activity of trasgenic plants and its control at different growth stages under field growth conditions. Also, we carried out Western blot and analysed the correlation between net photosynthetic rate and PEPC activities. Then treat transgenic plants with DCDP at filling stage for its fucntion. After harvested,we randomly selectedthe same number of transgenic plants and its control to survey its production traits. The study show the follwing results1. The results of PCR test showed that the genetic of transgenic plants were different and its positive rate was from2.76%-67%. Transgenic plants with the receptors were respectively Zhoumai19,01H186-20-24and17-1owned great stability, but transgenic plants with the receptors were respectively00H97-54-2-7and Zhengmai9023were unstability.2. After molecular detection, we select1-27-3、1-51-4、2-2-2and5-32-2as the material. We determined their flag leaf net photosynthetic rate, respectively, at jointing, heading and filling stage. The results showed that net photosynthetic rate of transgenic plants were all higher than that of the control. And at heading stage, transgenic plants reached the highest value, then were filling and joining stages. At filling stage, transgenic plants incresed21.4%,13.7%,13.5%and13.9%respectively compared with the control and the difference reached a significant level. Furthermore, the transgenic plant1-27-3was the best one.3. The results of PEPC activity assayed with transgenic plants and control showed that at different stage PEPC activity from high to low was the heading stage、the filling stage and the jointing stage and the trend was the same as the net photosynthetic rate. At heading stage, the PEPC activity reached the highest value and at filling stage the transgenic plants increased largely compared with the control with the increasement were2.4、2.3、1.3and1.6-fold, respectively. At jointing stage, there were no different between transgenic plants and untransformed. But, at heading stage, the PEPC activities of flag leaf were high than that of non-leaf organs. At filling stage, PEPC activities in flag leaf of transgenic plants could still maintain a high level and higher than other non-leaf organs. Activity of1-27-3was better than any other ones. The results of Westen blot analysis were the same with PEPC activities.4. The analysis of the correlation between flag leaf net photosynthetic rate and PEPC activities showed that there was significantly positive correlation and the correlation coefficient is0.8957**. We treated transgenic plants1-27-3and5-32-2with a specific inhibitor of the PEPC enzyme-DCDP. After treatment, the net photosynthetic rate of transgenic plants decreased significantly and it proved that the improvement of the net photosynthetic rate in transgenic plants were because of the importion of maize C4pepc gene.5. Transgenic plants After the harvest, we investigated the agronomic traits about transgenic plants and control plants.The results show that the yield traits of transgenic plants with different receptors were different.The yield traits of transgenic line1-51-4and2-2-2were no difference compared with the control, but the traits of1-27-3and5-32-2were great higher than the control on the single stem weight, grain weight, single panicle weight and harvest index higher than the control plants and the difference was significant.The single panicle weight and harvest index compared of1-27-3increased9.5%and14.4%respectively,compared with the control. |
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