Studies On The Development Of Microstructure Submicroscopic Structure And Photosvnthetic Characteristics Of Flue-cured Tobacco Leaf With Full Aroma Style

In this experiment, paraffin method, electron microscope chemical method, thephotosynthetic apparatus are used to research Tissue structure, cell structure andPhotosynthetic Characteristics of full aroma style flue-cured tobacco in Henan.Theresults are as follows:1. The development rule of microstructure of flue-cured tobacco leavesDifferent varieties of different leaf positions of full aroma style flue-cured tobacco toachieve the corresponding leaf length and state standards, the corresponding age is notconsistent. The general rule of Leaf microstructure development for early tissue cellsarranged closely, intercellular space smaller, Each layer of cells are oval or long oval,Yunyan87、YuYan10、NC71mesophyll cells of layer number is7-9,,7-8. With theincrease of days after the occurrence of the leaf, palisade tissue and spongy tissuedifferentiation, Cell gap further expand, palisade tissue longitudinal elongation,consisting of a long cylindrical cell, spongy tissue cell layer number is6-8. Generalupper leaf palisade tissue cell long coarse, lower leaf palisade tissue cell is shortrelatively fine, middle leaf center. Spongy tissue cells begin to shrink degradation, cellgap developed, cells arranged loose; sponge tissue thickness, size (maturity) for theupper leaf> middle leaf> lower leaf. Leaf blade for hyper mature stage, cell structureof upper and lower epidermal incomplete， degradation of palisade tissue cells, cellshrinkage and spongy tissue increased further. Leaf blade thickness from leaf stage tofixed length has been on an upward trend, the leaf thickness of upper leaves>middleleaves> lower leaves. Palisade tissue and spongy tissue thickness variation and leafthickness is the same with the leaf thickness.The thickness of palisade tissue/spongytissue thickness ratio is Changes of serration shape, The change rule of Differentvarietiesn is consistent. The maximum is NC71of middle leaf (leaf length35-40cm), the minimum value is NC71of lower leaves (leaf length5-10cm). The thickness ofpalisade tissue/leaf thickness ratio is upper leaf> middle leaf>lower leaf. Thethickness of palisade tissue/leaf thickness Variation of ratio rises and then falls.spongy tissue thickness/leaf thickness ratio is on the contrary2. The development rule of Submicroscopic of flue-cured tobacco leavesAlong with age increase, nucleus shrunk, Leaf cell sap concentration with increasingmaturity of decreasing. The nucleus position increasingly close to the cell edge.Nuclear heterochromatin in deepened. The change mainly lies in the amount oftobacco mitochondria cristae. The number of mitochondria cristae number areobvious characteristics of energy metabolism. Early chloroplast thylakoidsclear,Stacked low-rise,But the average lengthen, The leaves in different position ofthylakoids length relationship is the lower leaf> middle leaf>.upper leaf;Yanyan87>Yuyan10>NC71. To the age of33d, the most complex and chloroplastsubmicroscopic of leaf. Specific performance in the grana lamellae, matrix is strong,multiple layers grana took relatively major proportion. Leaf blade into thephotosynthetic product rapid accumulation period, rapid accumulation of chloroplastinclusion.After the chloroplast began to lose its definition of the lamellar structure,layer between the discrete..3. Studies on the photosynthetic characteristics of flue-cured tobacco leavesThe net photosynthetic rate, stomatal limitation value, the stomatal conductanceincreased first and then decreased. Intercellular CO2concentration is on the contrary.The comparison between different parts of the net photosynthetic rate is the upper leaf> middle leaf>lower leaf;NC71>Yunyan87>Yuyan10.