Identifying And Molecular Marker Analysis In Bud Mutation Of ’kyoho’ Grape

The ’Kyoho’ grape is Eur-american hybrid variety. It is a mid-maturing variety. The variety has strong growth vigor, strong adaptability, better disease resistance and high cold resistance. The trial contrasted the bud mutation and non-bud mutation’s grapevine in morphology and molecular biology. To provide a scientific basis for the identification of the new varieties. The results are as follows:①The morphology differentiations are the colour of young shoot between the’Kyoho’grapevine with its bud mutation. The later is slightly red than the former.②The average panicle weight and average grain weight all increase significantly in bud mutation. Average grain weight increase about2g. The sugar content, acid content, total soluble solid, and Vitamin C content are remarkable different between the two varieties. The bud mutation’s sugar and Vitamin C content increases, however, acid content decreases. All these showed that bud mutation can improve fruit commerciality.③The bud mutation is earlier mature one week days than the normal variety in phenology. This can improve the fruit sales price, adviod the typhoon hazard and swap fruit, disease dehiscent fruit, and birds’harm which brought by rainy, increasing the quantity of the commodity fruit.④Using57pairs of SSR primers covering19chromosome of grape to amplify the two varieties’ genomic DNA in genetic background validation. Results show that the two materials have same amplified bands and don’t find polymorphic bands. It shows that they have similar genetic background.⑤9primers with stable amplification and rich polymorphism were used for amplifying genomic DNA of47grapevine cultivars. The result showed that ’Kyoho’, its bud mutation and other varieties are differences in the genomic DNA level. At ISSR molecular marker identification aspect,29primers and7pairs of primer combinations with stable amplification and rich polymorphism selected from50primers were used for ISSR amplification.168unambiguous bands were amplified from the primers. The primer BC825can be used to distinguish the normal plant and its bud mutation. This indicates that’Kyoho’and its bud mutation are differences in the genomic DNA level.