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Application Of Functional Species And Effect Evaluation In Microbal Fertilizers

Posted on:2014-02-01Degree:MasterType:Thesis
Country:ChinaCandidate:H J ChenFull Text:PDF
GTID:2253330401979667Subject:Agricultural resource utilization
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Functional species and their effects were the Kernel of microbial fertilizers. In this paper,application of functional species and effect evaluation were researched by the data information of fieldexperiment proceeded in the past. The type, quantity, composition, identification, rejuvenation andapplication of functional species were analyzed. Further field experiment, in which4species of bacilluswere used, was carried out for effectiveness evaluation. The results would be great meaningful to thescreening of good strains, development of industry and stability increases of application.In the past ten years, the strains of functional species used in microbial fertilizers were notrestricted to the traditional bacteria, such as bacillus, but extended to fungi, pseudomonas and rhizobium.The species were increased from41to140, and most of them were screened independently rather thanpurchase. Screening, identification, preservation of strains and other key technology had been attractedconsiderable attention. The manufacture of microbial fertilizers was changed from simple labor toautomation. Density of culture was increased by50%in recent years. Quality was promoted andshelf-life was prolonged. The effect and function of strains were both multiplied, due to a graduallyoptimized composition of spices, which was the key to the production quality and application effect.With the development of high throughput screening, and the application of good strains or multiplespecies inoculants with plant growth promoting, organic matter decomposing, bioremediation, microbialfertilizer production would upgrade and promoted continuously in the near future.In field experiments, single species inoculant and multiple species inoculant were used oncucumber, rice and other7kinds of crops. For single species inoculant, Bacillus subtilis, Bacilluslicheniformis, Brevibacillus laterosporus and Paenibacillus mucilaginosus were functionalmicroorganism, respectively. Bacillus subtilis and Paenibacillus mucilaginosus were composed asmultiple species inoculant. The results showed that,(1) the effects of the same single species inoculantwere different for various crops. For example, the growth rate for Bacillus licheniformis was range from10.4%to13.2%, and from10.5%to14.8%for Paenibacillus mucilaginosus.(2) The effects of the foursingle species inoculant on same crops were different with a growth rate from7.5%to15.8%, whichreached significant difference level, and Paenibacillus mucilaginosus was the best.(3) The multiplespecies inoculant on rice could increase the crop yield by16.1%, which was better than single speciesinoculant with a growth rate of10.3%for Bacillus subtilis and10.7%for Paenibacillus mucilaginosus.This was in consistency with tendency of multiple species for superimposed effect.(4) All the microbialfertilizers were effective for growth-promoting on tested crops.Above all, application effect of microbial fertilizers was highly relevant with the functional strains,especially in the aspect of the source, matching ability and composition. The results will further confirmthe leading role of functional strains in microbial fertilizers and its application effect.
Keywords/Search Tags:Microbial fertilizer, functional species, effect evaluation
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