| Dragon’s blood is a traditional precious Chinese medicine, which has a wide range of medicinal value. As resources of Dracaena are scarce, many researchers have conducted many studies on artificially-induced dragon’s blood. However, the efficiency which artificially-induced dragon’s blood is not enough.In this research, it had a quantitative analysis of induction efficiency on a variety of inducers which induced Dracaena to produce dragon’s blood. These inducers included24different chemical inducers,9kinds of plant hormones. Five inducers Ultrasol Magnit (a kind of fertilizer),oxalic acid, zinc sulfate, magnesium nitrate, leucine and odium4-nitrophoxides increased the yield of dragon’s blood in Dracaena cambodiana trees by2.52-,3.14-,2.49-,2.28-.2.02-and2.26times compared to that of the control. The plant hormones such as gibberellic acid and brassinolide increased the yield of dragon’s blood in Dracaena cambodiana trees by2.57-and2.64-times compared to that of the control. A mix of IAA and gibberellic acid, IAA and kinetin increased the yield of dragon’s blood in Dracaena cambodiana trees by2.83-and2.01-times.It found that upon the elicitation of Fusarium proliferatum, a potent elicitor of dragon’s blood, the PAL enzyme activity in the leaves and stems of Dracaena cambodiana and other two Dracaena spp. significantly increased, accompanying with the formation of dragon’s blood, indicating the involvement of PAL in the biosynthesis of dragon’s blood, And also found there was a relativity between flavonoid content in Dracaena and PAL activity in the formation of dragon’s blood. In this work, we for the first time cloned a partial cDNA of PAL gene (DcPAL1) from Dracaena cambodiana seedlings by RT-PCR. DcPALl is highly homological to other known PAL genes registered in GenBank.In this research, ITS sequences and trnL sequences were used as system classification tag of several Dracaenas. The study found that Dracaena clustering results based on different molecular classification is not the same. It also found, the number of variable sites of trnL sequence is less than ITS sequences, such result couse different clustering results. Several Dracaenas which produces dragon’s blood clustered into on category in the trnL sequence phylogenetic tree. A result of this study proves that chemical-induction is practicable, and preliminarily studied the induction mechanism of Dragon’s Blood, which provides us with new ideas of dragon’s blood production. Moreover, molecular taxonomy of several Dracaena species gives us a new inspiration on Dracaena classification and identification effciently. |
