Research On Trametes Versicolor Tissue Culture Technology And Subsequent Cultivation Quality In Hainan

At present, Trametes versicolor is in great demand on the market at home and abroad. As This product has impressive medicinal value, by improve crop yields, quality and effect of the content of effective components, and shorten the growth cycle, a higher theoretical value will come out. Firstly, To ensure the culture medium, the purification, production, preservation and extra application of Trametes versicolor, most suitable solid cultivation of Trametes versicolor Mycelium Culture medium are screened. Secondly, from the research on the foster technology in Hainan province, author attain the best to cultivate Trametes versicolor to make largest benefit.1.First part of article solid medium screening experiment is preliminary screening test6carbon sources,9nitrogen sources and10inorganic salts. Then,do concentration optimization experiments with selected carbon source, nitrogen source and inorganic salts. By three factors three levels orthogonal experiment, finally author get a best combination between carbon source, nitrogen source and inorganic salts concentration to make the versicolor mycelium growth in the best growth condition and the fastest growth rate. Conclusions:a suitable versicolor mycelium growth solid culture medium formula is:potato200g+glucose5g+MgSO43.5g+KH2PO43g+beef powder3.5g+AGAR20g+H2O1L.2. Cultivation using succeeded versicolor bag cultured, wood cultured in the soil, wood cultured in the greenhouse and wood cultured in the tree four ways. Through the versicolor fruit body growth and yield of comparative study, the results show that wood cultured in the soil in the newly unearthed from the covered by eggs. In versicolor mycelium germination time, full of bags and the sustained time of succeeded cultural versicolor were faster than the other two Duan Mu versicolor. The cheese is highest cultured in the tree grow versicolor rate aspect, followed by greenhouses planting versicolor succeeded, the lowest is wood cultured in the greenhouse. Aspects of zhi chao from most wood cultured in the tree, has cheese4tide; Followed by bag cultured versicolor succeeded, the Chicago for3tide; the tide Is the worst wood cultured in the greenhouse, only for2. The fruiting body yield from more to less respectively is:wood cultured in the greenhouse, bag cultured, wood cultured in the greenhouser.3.Versicolor on different cultivation way grow fruit body test analysis of heavy metals in the body, the final results show that the different planting way versicolor cafa heavy metal content in the body are within the scope of the national edible fungi limits of heavy metals, and are lower than wild versicolor for heavy metal content in the body.Enrichment of heavy metals arsenic and lead are more clearly for the different ways of planting versicolor. For mercury and cadmium, it is not obvious. What’s more, heavy metal in wood cultured in the tree versicolor body increase with the wave number. wood cultured in the greenhouse planting versicolor and succeeded last versicolor were characterized by wave as the increase of the number of gradually reducing heavy metal content in the body.4.Different planting patterns of Trametes Versicolor Polysaccharide Content Analysis in vivo shows that Nobu Oonzhiko body polysaccharide content was the highest, accounting for3.20%, followed by wood cultured in the tree,3.05%. Section wood cultured in the greenhouse cultivation of Trametes versicolor was3.04%.Bag cultured of Trametes Versicolor Polysaccharide content was the lowest,2.90%. The difference of polysaccharide content are likely caused by the difference between culture medium, growth environment and resources.