The Purification Of Trametes Versicolor Polysaccharides And Its Biological Activity On Potato Virus Y

Potato virus Y (PVY) is a kind of worldwide virus, sympotoms(Mosaic,deformity, dwarf, peeling) are caused by infecting tobacco (Nicotiana tabacum),potato (Solanum tuberosum), pepper (Capsicum SPP.), tomato (S.lycopersicum) andso on, the yield and quality of tobacco leaves has been influenced seriously bycausing veins necrosis in tobacco leaf.We have determined the antiviral activities of12kinds of fungipolysaccharide,We discovered versicolor polysaccharide have strong immuneenhancement effect,the application of versicolor polysaccharide can prevent theinfection of TMV and CMV, also improve the quality and production oftobacco.However, the extraction process of Trametes Versicolor polysaccharide stillexists fautiness, its resistance to PVY is unclear.To optimize the extraction process,the optimized methods were explored byselecting better versicolor strain, comparing the extraction methods between hot waterextraction and ultrasonic extraction, optimizing the mycelium fermentation conditions(medium composition, fluid volume, time).To provide the basis for the developmentand application of new fungicide-Trametes Versicolor polysaccharides, the anti-PVYactivity of Trametes Versicolor polysaccharides were explored. The inducibleresistance and the effect on the activity of defensive enzyme was determinated.The effect of prevention and deactivation on PVY were detected by half leafmethod on Chenopodium amaranticolor and the inhibition of replication by Leafdiscs method on Nicotiana glutinosa，the control effect to PVY was tested by potexperiment.It was confirmed that the mycelium growth of YZ-1is faster than YZ-2,the yieldof polysaccharide is higher, it’s much more time-saving and efficient for ultrasonicextraction, the optimum fermentation conditions for mycelium growth is85mL fluidvolume in number3culture medium for7days, in this condition,the dry weight ofmycelium is the largest.The Versicolor polysaccharide has a strong preventive effect to PVY, thepreventive effect reaches73.98%. The inactivation effect was28.79%and theinhibition rate of multiplication reaches40.25%after being treated by700μg/mLTrametes Versicolor polysaccharide. Treated by polysaccharides can induce theresistance of host, the inhibition rate was59.84%,also increase the activity of tobacco defense enzymes than the contrast. The potted experiment showed that TrametesVersicolor polysaccharide has evident protective activity,14days after inoculatingPVY, the protective effect and therapeutic effect of polysaccharide300×wererespectively73.33%、52.38%, higher than the contrast.These results indicated that Trametes versicolor polysaccharides is animmunopotentiator and has a strong inhibitory action to PVY, application in advancecan achieve better protection effect.