| In this study, using the different cotton varieties (strains) and M37(♂Xinhai20×♀Shuofeng1),M44(♂Junmian1×♀Liaomian18), series4population(♂Junhai1×♀Liaomian18)and10Qpopulation (♂Xinhai16×♀108Fu) as material, investigated the agronomic characters ofgrowingseason and harvest economic characters, and find out the related indicators of cotton verticilliumwilt resistance by adopting natural disease nursery method and stem injection; using differentresistance cotton varieties, and made paraffin sections on those tissues, observed the differenceson tissues transverse structure among cotton varieties with different resistance, and find out therelated indicators of cotton verticillium wilt resistance.Tries to find out the disease-resistant maleparent (Xinhai20) and susceptible female parent (Shuofeng1) of M37by using in indoor SSRmolecular marker.And try to find out the difference of Polymorphisms stripe of the resistantoffspring and infected offspring, and filter out the relevant SSR molecular marker.The study willprovide valuable theoretical foundation for the disease resistance identification system of cottonverticillium wilt and breeding resistant varieties.Conclusions are as following:1) In M37population, susceptible plants accounted for36.07%, M44groups, susceptibleplants accounted for67.80%, this indicates that M37population obviously higher than M44population on disease resistance; In10Q groups, the relative disease index and lint percentage wassignificantly negatively correlated, Correlation coefficient is-0.759, It can be seen as the diseaseintensifies, lint significantly reduced.2) Resistance in different varieties of upland cotton, the relative disease index and plantheight, per plant effective boll number was significantly negatively correlated,correlationcoefficients were-0.463and-0.473, the result shows that as the disease intensifies, plant heightand per plant effective boll number Significantly decreased; In series4population, the relativedisease index and plant height, per plant boll number showed a significant negative correlation,correlation coefficients were-0.396and-0.394, the result shows that as the disease intensifies,plant height and per plant boll number Significantly decreased; These agronomic traits can beidentified as indicators of disease resistance.3) Disease resistance in different cotton varieties before Inoculation, as the disease intensifies,average size of the root epidermal cells increased from the409.3μm2(Xinhai20) to1448.5μm2(Junmian1), the average cell area increased to692.8μm2-3726.4μm2after inoculation, the resultshows that the average area of susceptible epidermal cells increased after inoculation, thereby thenumber of per unit cell area reduced, the gap between cells enlarged and this favorable to the rapidgrowth of Verticillium mycelium.4) Through the use of16pairs of SSR primers to filter between the disease-resistant maleparent(Xinhai20), susceptible female parent and offsprings (Shuofeng1) of M37, among themprimers BNL1414and BNL3452Could amplify polymorphism differential bands, therefore, webelieve that these two pairs of SSR primers can be used as a screening marker of disease-resistantcotton material. |
