| Tomato(Solunam lycopersicum)alias tomato,It is one of the most economical vegetable varieties in the world.Tomato has high nutritional value and beautiful color.It is a commonly used food material and has medicinal potential.It is deeply loved by the public.Tomato germplasm resources are relatively abundant.People understand the genetic diversity of tomato germplasm resources by investigating and analyzing agronomic traits,and then select high-quality resources for variety cultivation.Good germplasm resources are the basis for the birth of high-quality varieties.The above determines the characteristics of the breed,and also determines the future fate of the breed.Among all the agronomic shapes of tomato materials,the size and shape of the fruit and other intuitive commodity characteristics directly affect the choices of consumers.Therefore,these intuitive phenotypic traits are very important.In addition,tomatoes are often threatened by various diseases during the production process.The quality and yield of tomatoes have been greatly affected.Usually,the conventional control measures for these diseases are chemical prevention,but this method cannot maintain the control effect for a long time,and some chemical substances can also cause serious pollution to the environment.Therefore,it is very important to cultivate tomato varieties that carry disease resistance genes.Germplasm resources carrying antigen genes are the primary task.In this experiment,field investigations and molecular marker identification techniques were combined to perform phenotypic statistics and disease resistance identification analysis on 291 tomato high-generation inbred line materials,and selected high-quality tomatoes with excellent main phenotypic traits and resistance to multiple diseases Germplasm resources lay the foundation for the selection and breeding of new tomato varieties.The main conclusions are as follows:1.The phenotypic survey results of main agronomic traits showed that the agronomic traits of 291 high-generation inbred tomato germplasm resources had certain differences.Among them,the coefficient of variation of tomato fruit shape was the largest,followed by single fruit weight,fruit shoulder color,ripe fruit color and Growth habits,the tested tomato materials have great potential for genetic improvement2.According to the different performance of the 5 main agronomic traits among 291 tomato varieties,the hierarchical method in R language was used for cluster analysis,and the 291 tomato materials were divided into three major groups.The first group type belongs to the vigorous plant growth.Tomatoes with mellow fruit,rich fruit color and small weight;the second group type belongs to the vigorous plant growth,the fruit is round and the ripe fruit color is pink or red,the fruit shape is rich,and the single fruit weight is between Ⅰ and Ⅲ;This type of group has larger single fruit weight,rich fruit color,lush growth and high consistency of fruit shape.3.For genes whose molecular marker detection system has not been established in the two laboratories of Ty-1 and Mi,the two markers to be verified for Ty-1 and the two markers to be verified for Mi were obtained by consulting the literature,verified by actual PCR,and compared the markers.Results,successfully screened out molecular markers with good identification effects for the above 2 genes,which can be used for subsequent material identification.4.Utilize the molecular markers of the 7 genes of Cf-5,I-2,Ty-2,Ty-3,Frl,Sw-5,Tm-1 that have been established for the detection system and the Ty for the detection system established in the previous test-1 and Mi a total of 2 genes molecular markers,291 tomato high-generation inbred line materials were screened,of which 1 material contained 5 disease resistance genes at the same time,and 4 materials contained 4 disease resistance genes at the same time There are 26 materials containing 3 disease resistance genes at the same time,100 materials containing 2 disease resistance genes at the same time,136 materials containing 1 disease resistance gene at the same time,and 24 materials without disease resistance genes.In addition,the above-mentioned materials have good fruit quality and good agronomic traits. |
