Study On The Interaction Between Selenium And Lead On The Growth Of Agaricus Bisporus

Edible fungus fruiting bodies are delicate texture, rich in nutrition, many of these species also has a variety of health care, the edible fungus has been more a favorite of consumers. But the problems of heavy metal content exceeding standord often occured in recent years, the quality and safety of heavy metal in endible fungus were widespread concerned.Five common edible fungi (Pleurotus ostreatus, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, Auricularia polytricha) was cultured in plate cultivation with mycelium Pb added. Compared with the absorptive capacity of the Pb in mycelium of various fungi, it’s found that Agaricus bisporus absorb most of Pb. Agaricus bisporus was choosed for two further experiments of fermentation and fruiting body cultivation. The research herein is to investigate the interaction of selenium and Pb on the growth of Agaricus bisporus under a certain concentration of Pb treatment with different concentrations of selenium by testifying change law of nutrient content, membrane lipid peroxidation and antioxidant reductase enzyme activity. Expected the study to explore the application prospects of reducing the edible fungus lead content by adding selenium.The main results of research are as follows:1. In plate culture test, the absorptive capacity of Pb in mycelium of five common edible mushiooms(Pleurotus ostreatus, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Auricularia polytrich) was explored, it’s found that Agaricus bisporus absorb most of Pb.2. Compared the Pb absorption capacity in mycelium of five common edible mushroom, Agaricus bisporus absorb the most and was choosen for mycelia fermentation test.7days later, changes of chemical composition and physiological indicators of fermented mycelia was analyzed to investigate the influence of interaction of selenium and Pb to the nutrition and metabolism in Agaricus bisporus.The results show that compared with the treatment group of Pb, the Pb content and MDA content decreased significantly in the selenium treated mycelium, while the soluble protein content, total sugar content and glutathione peroxidase activity increased significantly, it’s indicated that the interaction of Se and Pb is a kind of antagonism. It’s also found that with Se concentrations increased the MDA content first decreased and then increased, indicating that low concentrations of selenium can reduce membrane lipid peroxidation, but high concentrations of selenium will enhance membrane lipid peroxidation.3. Choosing Agaricus bisporus as an experimental object for fruiting body cultivation test, Changes of chemical composition and physiological indicators in fruiting bodies was measured to investigate the influence of the interaction between Se and Pb to the nutrition and metabolism in Agaricus bisporus.The results show that in the Pb cultured compost addition of Se can significantly reduce the Pb content and MDA of the fruiting body, while increasing the selenium content, total amino acid content, total sugar content, superoxide dismutase and catalase vitality, and it’s proved once again that the interaction of Se and Pb is a kind of antagonism. With the increase of selenium concentration, MDA content first decreased and then increased, SOD and CAT activity first increased and then decreased, it’s indicated that low concentrations of Se can enhance the vitality of anti-oxidoreductase and work together to reduce membrane lipid peroxidation, but high concentrations of Se will inhibite the activity of anti-oxidoreductase and deepened membrane lipid peroxidation.