The Effect Of Substrate Depth For Light-Green Roof On The Turf Quality Of SIX Sedum

In this study, Inter-Simple Sequence Repeat (ISSR) molecular marker, sequence-related amplified polymorphism (SRAP) molecular markers and amplified fragment length polymorphism(AFLP) molecular markers were used to detect the genetic diversity and constructed the fingerprints of9accessions of Cynodon dactylon. The assessment of genetic diversity and variety identification were conducted using SRAP markers in order to lay the theoretical and technical foundations for its variety identification, germplasm resources protection, hybrid parents selection, development and utilization. The main research contents and results were as follows:1.The genomic DNA of was extracted by a SDS method.RNA was removed from the solution with RNase, and DNA was purified. The purified genomic DNA was suitable forISSR, SRAP and AFLP analysis.2.According to the results of ISSR, thirteen primer produced54polymorphic bands, among which34bands were found to be polymorphic, averaged4.1bands per primer. The percentage of polymorphic bands in average was64.15%. The Nei’s genetic similarity coefficient of the tested accessions ranged from0.547to0.962, the range was0.415. It showed that the test materials have ample genetic diversity. Analysis of cluster showed that all the accessions could be divided into5groups when the genetics similarity coeffieient is0.806.Blackjat and Nan jing Bermudagrass clustered into one group; Riviera and Bermudagrass clustered into one group; Yang jiang Bermudagrass clustered into one group; Regent clustered into one group; Tifway(T-419), Tifeagle, Tifdwarf clustered into one group.3.The results of SRAP showed that ten primer pairs produced230polymorphic bands, among whieh154bands were found to be polymorphic, averaged15.4bands per primer pair. The percentage of polymorphic bands in average was66.96%. The Nei’s genetic similarity coeffieient of the tested accessions ranged from0.606to0.867. The9accessions were classified into five major groups when the genetics similarity coeffieient is0.778. Blackjat and Yang jiang Bermudagrass clustered into one group; Bermudagrass clustered into one group; Riviera and Nan jing Bermudagrass clustered into one group; Regent clustered into one group; Tifway(T-419), Tifeagle, Tifdwarf clustered into one group.4.According to the results of AFLP, the following results were obtained. A total of1326bands were amplified by thirteen AFLP primer pairs from Cynodon dactylon genetic DNA, among which1212(98.74%) bands were found to be Polymorphic. The AFLP-based genetic similarity values among9Cynodon dactylon accessions ranged from0.469to0.797, and the range was0.328. Analysis of cluster showed that all the accessions could be divided into5groups when the genetics similarity coeffieient is0.652. Tifway(T-419), Tifeagle and Tifdwarf clustered into one group; Regent clustered into one group. Riviera and Blackjat clustered into the one group; Nan jing Bermudagrass and Bermudagrass clustered into one group; Yang jiang Bermudagrass clustered into one group.5.The fingerprint of nine Cynodon dactylon varieties was established by using ISSR, SRAP, ALFP markers.9Cynodon dactylon varieties can be identified by using the fingerprints established on the electrophoresis map of products amplified by primer of UBC810and UBC857. In this study25primer combinations were screened for9Cynodon dactylon varieties. Two SRAP primer combinations of me4/em6and me1/em6generated high polymorphic fragments, which can distinguish all of9Cynodon dactylon cultivars.9Cynodon dactylon varieties can be identified by using the fingerprints established on the electrophoresis map of products amplified by primer combinations of P7/M3.In a word, the results showed that it was feasible to assess the genetic diversity, analyze phylogenetic relationship and identify varieties of Cynodon dactylon using ISSR, SRAP, AFLP markers.