Localization And Express Of GLIS1and TCL1in Oocytes And Early Embryos Development Of Mouse

Totipotent means that the cell can differentiate into any type of the cells in the body. Mammalian development is started by a single fertilized egg, and the fertilized egg is recognized as having totipotent cells, which can develop into whole organisms and individual cells types. Recent studies have shown that early embryonic development, cell differentiation can be traced back to a four-cell embryo. Cell development to the8-cell stage, some cells will lose pluripotency and differentiation stage. After embryonic development to the blastocyst stage, the inner cell mass of the blastocyst stage are the main source of embryonic stem cells, a kind of totipotent cells.The entire organism development begins at the fertility moment, after fertilization, the fertilized egg coud be back to the totipotency state. The entire process so that by a single cell to develop into an entire organism become possible.In addition to early embryonic blastomeres, there are other types of totipotent cells, such as stem cells, tumor cells and iPS cells.GLIS1transcription factor belonging to the zinc finger protein, each finger has a conserved sequence GLIS1contains789amino acid residues, the DNA sequence5’-GACCACCCAC-3’binding, both activators of transcription inhibitor is also involved in cell proliferation, epithelial inflammation, psoriasis, birth defects, cancer, etc. The mRNA expression by PMA or gamma interferon induction. Maekawa et al.(2011) reported that GLIS1OSK factor co-transfection of human and mouse fibroblasts, significantly increasing the number of iPSCs clone, and reduce the number of non-iPSCs clone efficient in vitro pluripotent cells become possible. This suggests that we GLIS1in the the cell oocyte and early embryonic development may play an important role.Proto-oncogene gene TCL1(T cell leukemia) family of proteins first discovered the young in the translocation of human T-cell lymphoblastic leukemia (T-PLL). TCL1family of proto-oncogenes include106,114, and128amino acids, respectively, MTCP1, TCL1,, and TCLlb protein, and the predicted molecular weight of13(MTCP1),14(TCL1),15(TCL1b) kDa. These amino acids have a high sequence homology to the sequence. TCL1 with Akt interactive functional, TCL1has been shown to improve the activity of Akt kinase, TCL1can be used as the the Akt kinase auxiliary activator. It was also reported by PKB/Akt pathway, which regulate embryonic development in mouse zygotes. However, no report was available whether Tcll participate in the mouse embryo during early development, even in the PKB/Akt pathway involved in the swap.In this experiment, Kunming white mouse oocytes and early embryos as experimental material on Glisl and Tcll expression and distribution in the oocyte and embryo development process more systematic study. The entire experimental use of the immunofluorescence, RT-PCR technology and microinjection as the experimental technology.Results showed that:different expression level of protein of Glisl and Tell was observed in oocytes and early embryos in mouse. In detailed, from fertilized egg to the2-cell stage embryos, no difference on the expression level of Glisl and Tcll was observed both in nucleus and cytoplasm in mouse. However, after2-cell, the expression level of these two genes was decreased significantly till to blastocysts. The microinjection results of Glis1showed that the blastocyst rate was reduced after the injection of Glis1Mrna into the embryos, which might indicate that Glisl could influence the reprogramming of the embryos. But furthere studies are needed.