The Expression Of LRRN3in Middle And Late Mice Embryo Development

Objection:Observing the expression of the member of neural leucine-rich repeat protein family, LRRN3, in middle and late mice embryo development, to investigate its function in development.Methods:E9.5、E10.5、E12.5、E14.5and E16.5BALB/c mice embryos were gathered to extract RNA and protein. RT-PCR and western blot were operated to detect the expression level of LRRN3those mice embryos’brains. Meanwhile, in order to observe the expression of LRRN3in other tissues rather than brain, whole-mount mice embryos as well as organs from adult mice were embed in wax and made to slides for immunohistochemistry test.Results:Results of RT-PCR showed that there was merely no LRRN3expression existed in the samples extracted from E9.5mice embryo brains. However, LRRN3expressed in E10.5、E12.5、E14.5and E16.5groups while strongest expression was observed in E12.5with significant statistical difference compared with other groups(P<0.05). Results of Western blot showed that compared with other groups,there was little expression of LRRN3(P<0.05).LRRN3expressed stronger later on and reached the peak in E12.5group although it expressed less in E14.5and E16.5group. Immunohistochemistry staining was used in the slides of various developmental stages and besides brain, positive signals were found in nasal placode, intestines, liver, pancreas and dorsal root ganglia, while LRRN3expressed more specificity in adult organs.Conclusion:LRRN3presents sequential expression in the middle-late development of mouse embryos and participates in the development of multiple organs, such as brain, liver and pancreas which indicates LRRN3plays role during development not only in nervous system, but also other systems. The evidence mentioned above shows that LRRN3may be a gene associated with various systems of development.