Functional Analysis Of Auxin In Regeneration Of Physcomitrella And Cloning Of URO Homologous Genes In Physcomitrella Patens

Plant regeneration is an important part in plant growth and development. Asexual reproduction based on regeneration supports the development of Genetic Sciences. Thus the study of plant regeneration is of great value.In the study of the Auxin signal durning protonema and gametophore regeneration in P. patens, we find out that Auxin plays an important part in P.patens regeneration. Auxin signal peaked regularly after protonema disruption. And the signal level also increased steadly in the midvein of leaflets after the leaflets being setting apart from stems. The protonema can be discovered while the Auxin signal reaches their first peak. IAA, NPA or PCIB (IAA function inhibiter) can alter Auxin signal and the regeneration of protonema to a certain extent. All above implies that auxin, especially its transportation, may play an important part in P. patens regeneration.pH is also a crucial factor in moss development. We show here that the regeneration of Haplocladium capillatum and physcomitrella patens act differently under a series of pH-values media. Thus obtain preliminarily that different moss may vary in their mechanism in their response to pH-values.We also introduce URO to study the molecular mechanism of regeneration. URO alter IAA homeostasis through control the proportion of free and conjugated IAA. The understanding of URO in P. patens may provide some clues to reveal the function of Auxin in plant regeneration.