The Involvement Of FER And LLG1 In Auxin Signaling Pathway

Receptor-like kinases are involved in sensing external signals to regulate plant growth and development.A large number of receptor-like kinases present in plants,among which FERONIA(FER),a member of Catharanthus roseus RLK1-like(CrRLK1L)family,and its coreceptor glycosylphosphatidylinositol-anchored protein LLG1 play a crucial role in the regulation of plant growth and defense response.The results of this study showed that FER and LLG1 were required for various auxin-regulated processes,including the gravitropic response of hypocotyl and root,the formation of apical hook,the development of leaf vein and meristem.Meanwhile,to further study the relationship between auxin and FER,we observed considerable cytoplasmic retention of FER-GFP in auxin-deficient yucca1/2/4/6 mutant,and IAA treatments could effectively restore FER-GFP to the plasmalemma,indicating that auxin regulated the transport of FER from cytoplasm to PL.In addition,CrRLK1 L receptor kinases widely exist in plants from ancient species to more evolved species,therefore,we constructed Pp CrRLK1L2,PpCrRLK1L3 knock out mutants and RNAi-PpCrRLK1L4/5 mutant.As we didn't find obvious phenotypes in these mutants,further study is required to find out the function of the PpCrRLK1 Ls.