Functional Analysis Of Putative Lectin Gene AtLECL1in Arabidopsis Thaliana

As a kind of noenzyme and nonimmune genetic protein or glycoprotein, lectingenerallyresides in animals, plants and microbes. Lectin can identify carbohydratestereoselectively and combines with it noncovalent and reversibly. Furtherly, it can leadsome physiological effects with different organism cells, such as nitrogen fixation,guarding, and it also plays an important role in biotic stress and abiotic stress.On the other side, phylohormone is a kind of microamountant which is produced byplant itself, transported to definite site, and realize its physiological effect at a low level.Auxin(indole-3-acetic acid, IAA) is primarily identified among all the phylohormone, andit is also the most important endogenous hormones. Auxin mainly synthesises in eugenicmeristem regions, transports to definite site in gradient distribution by special carriersaiming at regulating cell mitosis and growth, and regulating plant development. Auxin isalso the most important hub in hormone interaction, and has its own function in regulationand coordination to other hormones. Our article investigates the lectin joins the rootdevelopment regulated by auxin.This article study the function of a new putative lectin gene, AtLECL1,based on thereverse geneticstheory and biochemistry, molecular biology method. We found that:atlecl1-5’ T-DNA is located in promoter region of AtLECL1, and the expression ofAtLECL1elevated about3times; the root length of atlecl1-5mutant just50%of WT after15days; under treatment of different phylohormone, we found that atlecl1-5insensitivetoIAA, for example, it’ s no fuction in mutant under the IAA concentration which rootgrowth of WT has already been promoted; by morphology observation, we found theconstructure of quiescent centrer in atlecl1-5changed; the expression of auxin polartransport genes PIN4and PIN7, auxin synthetic gene YUCCA and ASA1, and auxinrespond gene IAA1all have changed in atlecl1-5. We found AtLECL1is mainly located incellular membrane and asymmetrical distribution by eYFP reporter gene. All above, wepresume AtLECL1may participate the root development adjusting process of axuin.