Based On The New Nano Material Shell Of Palladium Glucose Electrochemical Biosensor Development

1. A novel Modified Glucose Carbon Paste Sensor Based on HollowPalladium and NickelA novel nonenzymatic amperometric glucose sensor based onhollow palladium and nickel modified carbon paste electrode wasdeveloped. The hollow Pd nanoparticles were synthesized andcharacterized by TEM. The modified carbon paste electrode wasprepared by mixing hollow Pd nanoparticles and graphite with acertain ratio, and then electrodepositing nickel film on the surface ofthe carbon paste electrode. This novel amperometric glucose sensorshowed good electrocatalytic activity towards the oxidation of glucosein alkaline solutions due to the presence of hollow Pd nanoparticles andnickel film. The modified electrode was surface-renewable andexhibited very sensitive current responses to glucose. The methodshowed good linearly for3×10-4M-9.3×10-3M glucose. The electrodewas verified by the determination of glucose, indicating that thismodified electrode has potential application in sensitive and selectivedetection of glucose. 2. A novel glucose sensor based on hollow palladium and glucoseoxidaseThe hollow Pd nanoparticles were synthesized and characterizedby TEM. A novel biosensor based on hollow palladium and glucoseoxidase immobilized on the surface of glassy carbon electrode forglucose detection was developed. The glucose can be determined bydetecting H2O2generated in enzyme reaction due to theelectrocatalytic activity of hollow Pd toward H2O2. Under the optimalconditions, a linear dependence of the catalytic current upon glucose concentration was obtained in the range of2.5×10-5M to2.7×10-3Mwith a detection limit of10μM （S/N=3）. The sensor also exhibitslong-term stability, good reproducibility and good anti-interferenceability. 3. A Novel Nonenzymatic H₂O₂Sensor Based on Hollow Palladiumand Au Nanoparticles Modified ElectrodeA novel nonenzymatic amperometric sensor based on hollowpalladium and Au nanoparticles for detection of hydrogen peroxide（H₂O₂） was developed. The hollow palladium nanoparticle（shollow Pd）were synthesized and characterized by TEM. The modified electrodewas prepared by electrodepositing Au nanoparticles （AuNPs） and thenmodifying hollow palladium on the surface of the Au-glassy carbonelectrode substrate. The electrocatalytic activity of the electrode（Pd/AuNPs/GC） toward H₂O₂was examined and compared tovarious modified electrodes, including GC, AuNPs/GC, Pd/GC viacyclic voltammetry. The results showed that Pd/AuNPs/GC displayedhigh electrocatalytic activity towards the reduction of H₂O₂due to thegood synergetic effect of hollow Pd and AuNPs on the reaction of H₂O₂.The response currents increased linearly with the concentration of H₂O₂in a wide range of2μM-216μM （R=0.9993） with a detection limitdown to0.2μM （S/N=3）. The sensor also exhibits good anti-interferenceability to electroactive molecules, including uric acid, ascorbic acid,glucose and ethanol.