Synthesis Of Pt-based Hollow Nanoparticles And Their Application In Non-enzyme Glucose Biosensors

Enzyme is easily denatured during its immobilization ptocedure, and the catalytic activity of GOx is susceptible to environmental conditions, which remain as problems for applications of enzymatic sensors. Therefore, non-enzymatic electrochemical glucose sensors provide a new approach for detection of glucose.Nano-crystalline particles is favorable material for constructing biosensors, due to their large surface area, high surface-activity and great efficiency of catalysis. The sensitivity, detection limit, the range of response and stability have been impoved with the special properties of nanomaterials. In this paper, we reported a non-enzymatic electrochemical glucose sensor based on Ag-Pt hollow nanoparticles which were synthesized by galvanic replacement. SEM images, TEM images, particle size distribution images, UV-vis Spectra, and XRD indicated the Ag-Pt hollow nanoparticles nanostructures. The electrochemical tests showed that Ag-Pt HNPs/CS/Au possess a large electroactive surface area and high electrocatalysis activity. The linear detection range was from 1~12 mM, response sensitivity is 7μA/mM, and detection limitation is 1.3×10-5 M. Meanwhile, the non-enzyme biosensor constructed by Ag-Pt HNPs/CS/Au electrode has a good stability and selectibity. Therefore, it will be more promising for Ag-Pt HNPs/CS/Au electrode to construct non-enzyme biosensor than the traditional enzyme electrode.Due to less expensive, low standard reduction potential, reaction more easily and approach more practical fro large-scale production of Cu2+/Cu, the present thesis was constructed Cu-Pt HBNPs/CS/Au electrode by galvanic replacement. We can confirmed the obtained particles were Cu-Pt hollow bimetal particles by SEM, TEM, particle size distribution, UV-vis Spectra, and XRD. The electrochemical tests suggested that Cu-Pt HBNPs/CS/Au possess a larger electroactive surface area and higher electrocatalysis activity. The linear detection range was from 0.5~14 mM, with response sensitivity is 8μA/mM, and detection limitation is 11.25μM. Meanwhile, the non-enzyme biosensor maked by Cu-Pt HBNPs/CS/Au electrode also has a good stability and selectibity. All this demonstrated that Cu-Pt HBNPs/CS/Au electrode showed a promising application in the determination of glucose.