| Purpose：YinQiao scattered from the qing dynasty wu Tang the article WenBingbian ", by honeysuckle, forsythia, design the ear, son, and mustard arctiumballoonflower, liquorice, mint, reed root, light bamboo leaves of nine flavourChinese traditional medicine, were playing cool XieBiao qingrejiedu, essien theefficacy, attending WenBing beginning, but not evil hot cold, or micro aversionto cold, headache, thirsty, sore throat and red tongue, pulse number card,traditional Chinese medicine is the representative of the method of Michaelessien cool XieBiao party. This study YinQiao powder were choose monarch,minister, adjuvant and make representative drug honeysuckle, forsythia, burdockthe son, and thorns the ear, balloonflower five arabidopsis medicine extract,and study the influenza virus in mice pneumonia treatment function andconcentration-response relations, this paper discusses the treatment of lunginflammation of antiapoptotic effect and its possible mechanism, for YinQiaoscattered more targets, multi-level and many link of the influenza virus actionto provide the theory basis and the experimental support.Material and method：1. The nasal vaccination influenza a viruses FM1strainsof the influenza virus establish infant mice pneumonia model. Application RT-PCRtechnology determination of influenza virus infection, and connecting with themouse lung tissue morphology, lung indices, the influenza virus in mice pneumoniamodel identification.2. Will YinQiao scattered five medicine to extract the way to fill the stomachflu medicine treatment mice viral pneumonia, observe the drug to mice are state,lung histopathology, lung index, the effects of the drug to observe differentconcentration in the therapeutic effect of influenza viral pneumonia.3. The immunohistochemical techniques testing various treatment groups of micein the lung tissue apoptosis related gene Bcl-2and Bax expression, observe different concentration YinQiao scattered five extract on flu medicine viralpneumonia mice lung tissue cell apoptosis influence.Results：1. The third day infection, the lung tissue can be measured in a flu virus RNAexpression, lung index increased significantly, by light microscopy in the lungtissue stroma venereal Richard change: the alveolar interval edema, inflammatorycell, the alveolar exudate serous cavity.2. The treatment group in mice mental state, activities, diet, breathing forimproving, group compared with model, are obviously improved. Light-microscopylung tissue lesions, the treatment group compared with model group weresignificantly reduced.3. YinQiao scattered five high dose of medicine group, the dose group of lowdose group, are different degree lower viral pneumonia mice lung index; The restof the dose of Chinese traditional medicine group and ribavirin group compared,reduce lung index in mice without difference obvious (P>0.05).4. On the third day after infection, model group lung tissue to IVRNA expressionof detection; In normal group has not been detected, Chinese traditional medicinegroup and ribavirin group virus nucleic acid in are all lower than model group,and with significant difference (P<0.01); Compared with western medicine,Chinese traditional medicine high dose group has significant difference (P<0.05), and the other the viral nucleic acid dose group of expression level hasno significant difference (P>0.05),. The comparison between traditional Chinesemedicine treatment group, high dose group of virus nucleic acid levels lowerthan other groups, there exists significant difference (P<0.05)5. Immunohistochemical method detection, normal group can be detected a few Bcl-2,Bax protein expression, model group group compared with normal lung tissue Bax,expression, enhanced obviously (P<0.01); The Chinese traditional medicine groupand ribavirin group of Bax protein expression was significantly lower than themodel group, and Bcl-2protein expression above model group, with significant difference (P<0.01). And ribavirin group compared, high dose group oftraditional Chinese medicine in reducing Bax protein expression, improve Bcl-2protein expression, there are significant difference (P<0.05). The rest of thedose group of traditional Chinese medicine has no significantConclusion：1. The nasal vaccination flu virus can cause influenza virus success in micepneumonia model, reference lung index, observation organization form and usea PCR detection technology can RT appraisal influenza virus pneumonia model.2. Different doses YinQiao scattered five medicine extract all have reduce lunginflammation role in mice, and the Chinese traditional medicine group thetherapeutic effect of drug concentration and positively correlated with higherdose treatment group best effect.3. The influenza virus infection in mice induced lung tissue can happen cellapoptosis, apoptosis related gene Bax, Bcl-2in mice flu viral pneumonia in thelung tissue are abnormal expression, may and Bax, Bcl-2gene expression aboutunbalance; YinQiao scattered five medicine extract can significantly inhibitedinfluenza viral pneumonia lung tissue cell apoptosis, possibly through adjustingBax/Bcl-2about balance. High doses of the regulation of apoptosis of even moresignificant.4. On the third day after infection, model group lung tissue to IVRNA expressionof detection; In normal group has not been detected, Chinese traditional medicinegroup and ribavirin group virus nucleic acid in are all lower than model group,and with significant difference (P<0.01); Compared with western medicine,Chinese traditional medicine high dose group has significant difference (P<0.05), and the other the viral nucleic acid dose group of expression level hasno significant difference (P>0.05),. The comparison between traditional Chinesemedicine treatment group, high dose group of virus nucleic acid levels lowerthan other groups, there exists significant difference (P<0.05)5. Immunohistochemical method detection, normal group can be detected a few Bcl-2, Bax protein expression, model group group compared with normal lung tissue Bax,expression, enhanced obviously (P<0.01); The Chinese traditional medicine groupand ribavirin group of Bax protein expression was significantly lower than themodel group, and Bcl-2protein expression above model group, with significantdifference (P<0.01). And ribavirin group compared, high dose group oftraditional Chinese medicine in reducing Bax protein expression, improve Bcl-2protein expression, there are significant difference (P<0.05). The rest of thedose group of traditional Chinese medicine has no significant... |
PDF Full Text Request