Regulator With Chinese Traditional Medicine Take Resistance While Tuning Methods Such As The Study On Prevention And Control Of Dysbacteriosis Caused By Antibiotics

Objective： This topic applied Chinese medicine microecological modulator2011,adopting the style of simultaneous administering antibiotics and the modulator; firstadministering the modulator and later the antibiotic; first the antibiotic and later the modulator,to research on mouse intestinal microflora dysbiosis caused by antibiotics; aiming towards thecorrect clinical usage of antibiotics and thereby helping in reducing the side effects of antibioticsand in the process providing a scientific basis for the usage of antibiotics. During this research,in addition to the traditional culture methods, the fluorescence in situ hybridization (FISH) wasalso used to detect the changes in the intestinal microflora, improving the accuracy of the speedof detection and the changes and the changes that occur in the different species of the intestinalmicroflora.Method： Used130pure Kunming mice both male and female weighing18-22g. Themice were randomly divided into groups after which20mice selected as the normal controlgroup. The other110mice were then orally administered Lincomycin hydrochloride for7daysto induce intestinal microflora dysbiosis. After that, from the three perspectives of simultaneousadministering antibiotics and the modulator; first administering the modulator and later theantibiotic; first the antibiotic and later the modulator, the110mice were orally treated withChinese medicine polysaccharide, Oral Live Bifodobacterium preparation and a mixture of boththe Chinese medicine polysaccharide and the oral live bifidobacterium for7days, after whichthey were sacrificed. Intestinal contents, blood, liver and intestinal mucosa were then extractedand separately cultured after which indexes like FISH stool test, bacterial translocation,volatile fatty acid content of the intestinal contents, blood endotoxin and serum IL-2, intestinalmucosa SIgA were taken.Results： In this experiment, the use of Lincomycin hydrochloride to create intestinaldysbiosis in the pure Kunming mice was a success; compared with the normal control group,the model group had a decrease in the number of probiotic bacteria; there was an increase innumbers of E. coli, and the enterococci, while there was an increase in the blood endotoxinlevels also; however, there was a decrease in blood plasma IL-2, intestinalmucosal SIgA contents, all in all, a high significant difference (p<0.01). Compared with themodel group, the first modulator and later antibiotics group had an increase in the numbers ofthe probiotic bacteria, with a decrease in numbers of both E. coli and the enterococci, there wasalso a decrease in the blood endotoxin levels, with an increase in plasma IL-2and intestinalmucosa SIgA with a significant difference (p<0.05); Compared with the model group, thesimultaneous administering antibiotics and the modulator group had an increase in the numbersof the probiotic bacteria, with a decrease in numbers of both E. coli and the enterococci, there was also a decrease in the blood endotoxin levels, with an increase in plasma IL-2and intestinalmucosa SIgA with a significant difference; moreover, a comparison between the groupsshowed that the Prebiotics and synbiotics had a different and statistically significant differenceeffect on the probiotic bacteria (p<0.05); compared with the natural recovery group, first theantibiotic and later the modulator group had an increase in the numbers of the probiotic bacteria,with a decrease in numbers of both E. coli and the enterococci, there was also a decrease in theblood endotoxin levels, with an increase in plasma IL-2and intestinal mucosa SIgA with ahigh significant difference (p<0.01).Conclusion：Lincomycin hydrochloride can be successfully used to create intestinalmicroflora dysbiosis. Synbiotic number2011fosters the growth of normal intestinal flora, has aregulatory effect on intestinal flora dysbiosis, improves immunity and can play a role in theprevention of intestinal infections. First administering the modulator and later the antibiotic canprevent intestinal microflora dysbiosis caused by antibiotics; the use of the mixture of both theChinese medicine polysaccharide and the oral live bifidobacterium in the simultaneousadministering antibiotics and the modulator group had a better effect than the single usage of theOral Live Bifodobacterium preparation; comparing the natural recovery group, and first theantibiotic and later the modulator group, there were significant differences, and moreover themixture showed the best results.