Pharmacognosy Study Of Tibetan Medicine "the Color"

"ESe"(Tibetan] is the Ganzi Tibetan Autonomous Prefecture Tibetan Autonomous Prefecture of Tibetan folk medicinal materials traditionally used blindly," ESe from the dry leaves of Malus toringoides (Rehd) Hughes and Malus tiansitoria (Batal.)Schneid. Only contained in the drug "crystal mirror Materia Medica" ESe gray as the moon is generally beautiful, fruit such as precious stones are generally beautiful, and butter tea tomatoes take to cure indigestion and liver disease ".In this article," ESe " for the first time had a more detailed morphological and histological studies to identify the main distinguishing features of its original plants, medicinal properties, and organizational structure. Original plant morphology, mainly in terms of leaf shape, size, coat the situation, the number of stamens, ripe fruit shape, size, color and calyx depression and other identification; medicinal properties, mainly from the color, leaf split to identify; on the microscopic characteristics of the petiole cross-section from the main vein vascular arrangement, shape and size of the parenchyma were identified, mainly in powder microscopy of epidermal cells, stomatal and non-glandular hairs, catheters, clusters of calcium oxalate and cubic differentialThis is the first time to use the system chemical composition of pre-experimental methods of Tibetan Medicine " ESe " of a more comprehensive pre-experiment. Through its water extract, alcohol extract and petroleum ether extract of the chemical composition of the pre-experiment, to determine the water extract contains amino acids, polysaccharides chemicals, alcohol extract flavonoids chemicals.On the basis of chemical composition in the early pre-experimental and flavonoids extraction, we are the first to complete Tibetan Medicine "ESe" and chemical composition of flavonoids extraction, separation, identification of research. We also did a survey of Tibetan Medicine "ESe" in flavonoids extraction, separation, identification of research. And isolated two compounds, manager of constant measurement and the H-NMR, the C-NMR spectroscopy to determine is the root quercetin and quercetin.For the first time, we were trying to find the suitable time and collection period of "ESe", drying rate,"ESe" extract, flavonoids dynamic changes, the results show that the accumulation of biomass, extract, total flavonoids, rootthe total amount of quercetin and quercetin are peaked in mid-July to the end of July, and Russian color and lush growth, yield, starting from the herbs of the inherent quality and production point of view, that the "Russian colorsuitable harvesting period in mid-July to the end of July. The study provides a scientific basis for Russian color herbs harvested period. For the first time established a TLC identification of the Tibetan "Ese" of flavonoids. Ethyl acetate-butanone-formic acid-water (5:3:1:1) as solvent, good separation results, after the commencement of the Rf value is moderate, spots clear. Were not10batches of change Yehai Tang and flower the Ye Haitang samples TLC test results show that the chromatogram of the test, the corresponding position on chromatography with reference substance, was the same yellow fluorescent spots. The identification of Tibetan Medicine "Ese" identification of hyperoside has better adaptability, and good reproducibility.According to the Chinese Pharmacopoeia2010edition, an appendix item under the method of Lot20"Ese" medicinal samples of water, total ash and acid insoluble ash of the check, the measured data, combined with commodity situation and production of practical, prepared itslimit not more than8%moisture; total ash should not exceed8%; acid-insoluble ash not more than0.5%.For the first time, we established the "ESe" extract determination, with60%ethanol as the solvent, the hot-dip method for the determination of the measured data based on20batches of Tibetan medicine samples of Russian color, combined with the actual production, the development of the limits of its content forshall not be less than20%.For the first time, we established the total flavonoids of the "ESe" UV-visible spectrophotometric determination of methods and phloretin, quercetin HPLC method and the determination of20batches of samples. Based on the measured data, combined with the actual production, formulation standards of its limits:This product is the total flavonoids (rutin) variable Ye Haitang not less than1.82%, and the flowers Ye Haitang not less than1.62%. Containing quercetin and phloretin the total amount of not less than0.049%.The first time on two different sources of Tibetan Medicine "ESe"quality evaluation, combined with the drying rate, extract, total flavonoids, phloretin, and quercetin content indicators, using the weighted average of variable Ye Haitang andflower Ye Haitang varieties evaluation and comparison of the10batches of variable Ye Haitang and10batches of flowers Ye Haitang dried leaves of the test indicators, initially to determine the variable Ye Haitang Begonia optimum yield and internal quality.According to the above research results, we made the first set of Tibetan medicine "ESe" quality standards [draft].