| [Objective]To investigate hypoxia induced cardiomyocytes apoptosis and the expression of the protein of Bcl-2, Bax and NF-κB, as well as the protection effect of17β-estradiol on the hypoxic cardiomyocytes.[Methods]Cardiomyocytes were isolated from the hearts of1-2-day-old rats (Sprague-Dawley), then we cultured them, we treated the cells when the cells were in good condition, intervented them with17β-estradiol and stimulated the cells under hypoxia environment. Cells were divided into four groups, respectively, Cl (control group), C2(simple17β-estradiol group), H1(simple hypoxia group)、H2(hypoxia and17β-estradiol group). The groups of C2and H2were treated with17β-estradiol for24h, then the groups of H1and H2were treated in hypoxia environment (5%CO2+95%N2) for12h, and then harvested them for the next experiments. We used the method of MTT to assay the impact of hypoxia on cardiomyocytes viability and the inhibition rate and the protection of17β-estradiol on hypoxic cardiomyocytes. Flow cytometry was used to detect the apoptosis of the cardiomyocytes. The expression alternation of protein of Bcl-2, Bax and NF-κB were measured by Western Blot. The expression alternation of mRNA of Bcl-2and Bax were measured by Real-Time PCR.[Results] Hypoxic decreased the viability of the cardiomyocytes, and inhibited them,10-8,10-’mol/L17β-estradiol can improve cell viability and reverse the inhibitory effect of hypoxia on cardiomyocytes. Hypoxia aggravated the apoptosis of the cardiomyocytes, increased the expression of protein of Bax and NF-κB and mRNA of Bax, decreased the expression of protein of Bcl-2and mRNA of Bcl-2. Compared with the group of single hypoxia, the group of hypoxia which was pretreated with10"’mol/L17β-estradiol, the the expression of protein of Bax and NF-κB and mRNA of Bax were decreased, while the the expression of protein of Bcl-2and mRNA of Bcl-2were increased.[Conclusion] Hypoxia had damage and inhibition effect on cardiomyocytes, and aggravated the apoptosis of the cardiomyocytes, decreased the expression of protein Bcl-2and mRNA of Bcl-2, the increased the expression of protein of Bax and NF-κB and mRNA of Bax.17β-estradiol can reverse the inhibition of hypoxia on cardiomyocytes, this protective effect maybe by reducing the expression of NF-κB and then lead to increase the expression of Bcl-2mRNA and decrease the expression of Bax mRNA. The aim of the present study was to evaluate the relation between fat mass (FM), fat free mass (FFM) and ventilator function in children and adolescents.1174healthy children and adolescents (583males and591females) aged10-18years were selected from Heilongjiang Province through random sampling by means of questionnaire and physical examination, and measured for height, weight, waist to hip ratio (WHR), FM, FFM and ventilatory function. The data were analyzed by means of independent-samples t test, Pearson correlation analysis and multi-factors regression analysis. Regardless of sex, an independent positive correlation was found (P<0.001) between age and FFM index (FFMI). FM index (FMI) correlated negatively with age in males (P<0.001), but positively with age in females (P<0.001). Regardless of sex, FFMI correlated positively with forced vital capacity (FVC), forced expiratory volume in one second (FEV1), peak expiratory flow (PEF), forced expiratory flow at25%of forced vital capacity (FEF25%), FEF50%,and maximal mid-expiratory flow (MMEF)(P<0.05), while negatively with FEV1/FVC (P<0.01). FFMI was correlated positively with FEF75%in males (P<0.05), but not correlated in females. In males, FMI correlated negatively with FEVI, FEV1/FVC, PEF, FEF25%, FEF50%, FEF75%and MMEF (P<0.05), but not correlated with FVC. No correlation was found between the ventilator function indices and FMI in females. Except FEV1/FVC and FEF75%in males, the effect of FFMI in predicting ventilatory function was higher than FMI regardless of sex. Moreover, the predicting effect of FFMI was higher in males than that in females. Growth spurt of lung function occurred in the ages of12-15years in males, while in the ages of12,13and18years in females. During the period of growth spurt of lung function, regardless of sex, the effect of FFMI in predicting the lung function was higher than that of age. In conclusion, regardless of sex, FFMI correlates positively with ventilatory function, as a reflection of muscle mass. The effect of FFM in predicting ventilatory function is higher in males than that in females. FM correlates negatively with ventilatory function in males, but not in females. The rapid growth of height and FFM are possibly the main reasons for growth spurt of lung function. |
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