Heat Phlegm Decoction Youth Essential Hypertension Phlegm Heat Indicates The Curative Effect And Metabonomics Studies Certificate

Objective:To observe the curative effect of Chinese medicine Qing Re Hua Tan (QRHT) Tang in youth essential hypertension (YEH) patients with abundant phlegm-heat. And based on’H-NMR metabolomics, combining Principal Components Analysis (PCA) and Partial Least Squares Discriminate Analysis (PLS-DA) to explore the serum endogenous biomarker substances of YEH patients with abundant phlegm-heat and observe the effect of QRHT Tang in those serum endogenous biomarker substances after4weeks treatment. Provide objective basis for diagnosis and TCM treatment of EH patients with abundant phlegm-heat syndrome.Methods:33selected cases all came from Guang’anmen Hospital, China Academy of Chinese Medical Sciences out-patients with YEH patients and differentiated by two associate chief physicians with abundant phlegm-heat syndrome. All the patients were treated by QRHT Tang for4weeks. During the treatment, patient measured his/her blood pressure three times a day and reported it to the doctor when he/she.came to hospital meanwhile the doctor asked the patient about the symptoms and finish the Case Report Form (CRF). Select12male YEH patients and12male healthy young people. All serum samples were detected by’H-NMR and research the differences of metabolites by PCA and PLS-DA.Results:1. Curative effect of QRHT Tang on blood pressure:blood pressure began to drop at the very first week. After continuous4weeks’treatment, there were23markedly effective cases and8effective cases; the total effective rate was93.93%.2. Curative effect of QRHT Tang on syndrome and symptoms:syndrome score began to drop at the very first week. After continuous4weeks’treatment, there were26markedly effective cases and5effective cases; the total effective rate was93.93%. QRHT Tang can improve symptoms.3. The serum substances of YEH patients with abundant phlegm-heat and the healthy people can be distinguished by’H-NMR metabolomics methods. The serum endogenous biomarker substances of YEII patients with abundant phlegm-heat are:LDL/VLDL, N-Ac, O-Ac, lipid, HDL, PtdCho, Lactate, Alanine, Cho/Pcho, GPC, Glucose,3-HB, Citrate, Glutamine.4. After4weeks’treatment, the serum substances can be distinguished between the YEH patients with abundant phlegm-heat and the healthy people. The serum endogenous biomarker substances tendered to the healthy people after the treatment.Conclusion:1. Chinese medicine QRHT Tang can effectively reduce the patient’s blood pressure and the symptoms score, improve the quality of life.2. There are metabolic disorders in YEH patients with abundant phlegm-heat. The content of Glueose, Lactate and Citrate in those patients are lower than health people shows the carbohydrate metabolism disorders. The content of LDL/VLDL, lipid and UFA in those patients are higher than health people, while the content of HDL, PtdCho, Cho/Pcho, GPC and3-HB in those patients are lower than health people shows the lipid metabolic disorders. The content of Alanine and Glutamine in those patients are lower than health people, while the content of N-Ac and O-Ac in those patients are higher than health people shows the proteometabolism disorders.3. Chinese medicine QRHT Tang can effectively change the metabolic disorders in YEH patients with abundant phlegm-heat. QRHT tang can appropriately increase the content of Glueose, Lactate, Citrate, HDL, PtdCho, Cho/Pcho, GPC,3-HB, Alanine and Glutamine. It also can appropriately decrease the content of LDL/VLDL, lipid, UFA, N-Ac and O-Ac. Provide objective basis for its curative effect.