The Experimental Study Of Analgesic Effects And Anticancer Of Pileostegia Viburnoides

Objective:Through the antitumor and analgesic experiments, to explore Pileostegia vibumoides inhibition and analgesic action of tumor.screened with clear anti-cancer pharmacological active substances and analgesic action of extraction of parts. In order to further study Pileostegia vibumoides antitumor surfactant analgesic action provide pharmacology basis material base.Methods:The growth inhibition was analyzed by MTT assay and cell colony measurement technique in human cervical cancer Hela cells and stomach carcinoma SGC7901in vitro. The models of mice bearing transplantation S180sarcoma were established,with thymus index,spleen index, and tumor weight as the observation indicators, to explore the inhibiting tumor action of four kinds of extracts from Pileostegia vibumoides on bearing S180cancer mice and its immune regulation action.With bearing S180cancer mice as the research object,thymus index, spleen index, and tumor weight as the observation indicators, discusses the effect of four kinds of extracts from Pileostegia vibumoides and cyclophosphamide combined; With S180ascites mice as the research object, observe Pileostegia vibumoides four extracts the extension of life cycle in mice,Pileostegia vibumoides utility parts of analgesic effects were optimized by the tenderness test and acetic acid writhing in mice. To clear Pileostegia viburnoides the effect of analgesic and anticancerResults:①MTT assay and cell colony measurement technique were indicated, The growth of all tumor cells were inhibited by Pileostegia viburnoides extracts of petroleum, ethyl acetate and water fractions (P<0.01).②Through the Tumor weight can be learned,Pileostegia viburnoides extracts of petroleum and Ethyl acetate, can Inhibits the growth of tumor mass. Compared with the positive drug cyclophosphamide, Pileostegia viburnoides extracts of petroleum and Ethyl acetate have different degree of protection to thymus and spleen (P<0.01),Pileostegia viburnoides four kinds extracts and cyclophosphamide combined can enhance antitumor effect (P<0.01)③Pileostegia viburnoides extracts of petroleum andethyl acetate can obviously prolong the S180ascites mice survival time.④Pileostegia viburnoides extracts of petroleum and Ethyl acetate can prolong the pain threshold value and inhibit acetic acid-included writhing of mice, shows a certain effect of analgesic.