PM_2.5 Have An Acute Inflammatory Response On The Cardiovascular System Of Diabetic Rats

Objective: Particulate matter is one of the major risk factors forcardiovascular diseases. There is evidence that respirable particulate matter ofair pollution can increase the morbidity and mortality of cardiovasculardiseases. By rapidly into the blood circulation, particulate matter can directlycontact with the vascular endothelial cells, induced inflammation or injury,promoted thrombosis, affected the stability of atherosclerotic plaque.Particulate matter have a high degree of correlation with myocardial ischemia,arrhythmias, increased blood pressure, decreased heart rate variability （heartrate variability, HRV） and the increase of circulating markers prompted theinflammation and thrombosis in peripheral blood. Coronary heart disease（CHD） is the leading cause of death and hospitalization among adults65ormore years of age, therefore the prevention of heart disease is becoming animportant research goal. Numerous epidemiologic studies have shown thathospitalization rates and mortality of cardiovascular disease are generallyrelevance with outdoor （ambient） air pollution. Diabetes is a common disease,and the major hazards are the complications of the major organs and bloodvessels of heart and brain, such as coronary heart disease, diabetic nephropa-thy, macrovascular disease and microangiopathy. With the number of diabetesincreased year by year, it is a hotspot of the early prediction and treatment fordiabetes. Adiponectin is peptides of secreted by fat cells, abundant in plasma（1.9-17.0mg/L［1］）. Adiponectin can enhance insulin sensitivity, antiinfla-mmatory, antiartery atherosclerosis, etc. So it is provided the new ideas anddirections for the prediction and treatment of diabetes and its complications.In this paper, we used the intratracheal instillation of different concentra-tions of particulate matter, and observed changes in rat inflammatorycytokines, further understanded that the particulate matter was a potential risk factor for cardiovascular disease. And also further explored that the particulatematter had hazardous greater for the individuals with cardiovascular riskfactors than healthy individuals, and different concentrations of particulatematter effected on the cardiovascular system in varying degreesMethods: Total60rats were chosen, including30male Wistar healthyrats and30male Wistar diabetes rats. These rats were divided into3groupsrandomly: saline group, the low-dose group （7.5mg/kg） and the high-dosegroup （15mg/kg）, exposed by intratracheal instillation of law, in each grouprats were randomly divided into two groups, the rats were respectivelysacrificed after24h or72h,10%chloral hydrate intraperitoneal anesthesia,femoral artery bled to collect blood to detect the WBC, CRP, IL-10, NOS,adiponectin levels, rats myocardial carotidartery, HE staining vascularendothelial injury and myocardial tissue neutrophil infiltration.The date was analysed by SPSS13.0statistical software,P <0.05conside-red statistically significant. Measurement data were mean±standard deviationsaid, count data were expressed as a percentage. Comparison of the each groupmean were tested by multi-factor factorial design analysis of varianceResults: By using excel2003and SPSS13.0statistical software foranalysis, and using multi-factor factorial design analysis of variance test,compared with the saline group, the levels of WBC, CRP, IL-10, NOS,adiponectin were difference （P<0.05）,and the difference was statisticallysignificant; And high concentrations of particulate matter instillation groupwas significantly higher than the low-dose group （P<0.05）, with a statisticallysignificant; The increase of these indicators was more obvious in diabetic ratsgroup than in the normal rats group, P <0.05, with a statistically significant.Conclusions: After intratracheal instillation of different concentrationsof PM_2.5, the system would produce the inflammatory response, and with theparticulate matter concentration, the inflammatory response were more prono-unce.In the experimental, diabetic rat tracheal droped of PM_2.5, the degree ofchange of the monitoring indicators were more than normal Wister rats, and adiponectin levels were significantly lower, indicating that the particulatematter was more serious impact on the cardiovascular system of diabeticrats,and may accelerate the progress of diabetes...