| Objective:The rat model of constipation-predominant irritable bowelsyndrome was induced by intragastric administration of ice water. Gave MaZhi Jiang Zhuo, compound aloe capsules and cisapride respectively to themodel rats to observe the general condition, body weight, stool water content,small intestine charcoal suspension pushing rate, the expression of5-HT andpathological changes in intestinal tissues of the model rats, and to explore therelationship between5-HT and constipation-predominant irritable bowelsyndrome, and the intestinal protective effect as well as its possiblemechanism of Ma Zhi Jiang Zhuo. This may provide experimental basis ofChinese medicine in the clinical treatment of constipation-predominantirritable bowel syndrome.Methods:45healthy male wistar rats of clean grade, weighing140±20g,each rat was caged and fed independently. Adaptive feeding lasted for oneweek, this was the baseline period. Rat feces and stool water content wereobserved before experiment and those normal ones could be used in theexperiment. In the following week,45healthy male wistar rats were randomlydivided into the model group and the normal group (N). The model group,including37rats, was given ice water while eight normal ones were givennormal food and water. Gave ice water mixture (0-4℃saline) to the modelgroup (i.g.)2ml once per day for14days. In order to eliminate the impact ofbiological rhythms, the rats were all gavaged at8:30am. After modelingsuccessfully (success criteria: fecal grains, fecal morphology and watercontent change), the model rats were randomly divided into four groups:cisapride group (X), compound aloe capsules group (Z), Ma Zhi Jiang Zhuogroup (F), and model group (M). Medicines were delivered one week after successful modeling, and calculated the dosage according to body surface area.X rats were given cisapride3.6mg/kg/d (i.g.), Z rats were given compoundaloe capsules0.36g/kg/d (i.g.), F rats were given Ma Zhi Jiang Zhuo6.3g/kg/d (i.g.), M rats were treated with distilled water2ml/d (i.g.). N ratswere fed normally. After the last administration, the rats were fasted for24h,but were given normal drinking water, preparing to take specimens.30minutes before the experiment started, each rat was gavaged4ml anthracotic.All rats were decapitated, and then laparotomized quickly to remove allgastrointestinal tissues, and measured the length from the pylorus to thefront-end of the carbon and the distance from the pylorus to the ileocecal.Then quickly took the ileocecal, colon (5cm from anus), small intestine (5cmfrom ileocecal), each0.5cm, and immediately rinsed with saline, fixed withl0%neutral buffered formalin. Took three specimens of intestinal tissue fromeach group, put them under optical microscope to observe their pathologicalchanges, and investigated the expression of intestinal5-HT usingimmunohistochemical methods.Results:1General states of the ratsNormal rats had good spirit, quick response, the eyes of God, strongmuscles, shiny and supple fur. They had straight tails, no deformity, as well asnormal diet. The stool was grain shape, about0.9cm, tawny, soft, and easy tobreak apart. The urine was normal. Compared to the normal group, the rats inmodel groups were a bit nervous in spirit and overreact, the fur was slightlydry and frizz. The stool was mostly small soybeans like and sometimes mixedwith the grain shape stool. It was about0.4-0.6cm, taupe or gray, harder thannormal, and could not be broke apart individually. During the different stagesof the experiment, there was no death of rats and no diarrhea during the entireexperiment. In the modeling process, a rat appeared primary cataract in theright eye, a rat was scratched by cage when escaping. In order to reduce theimpact factors, they were removed from the model groups.2Stool water content The results showed: after14days’ intragastric administration of ice water,the stool water content of model rats was significantly decreased than normalrats, this proved modeling successful. The four model groups(X, Z, F, M)showed no significant difference (P>0.05). After14days’ administration ofthe drugs, the stool water content of groups which were given compoundaloe capsule, cisapride and Ma Zhi Jiang Zhuo treatment respectively wassignificantly higher than those of the model group(P<0.05). There was nosignificant difference between treatment groups (P>0.05).3Small intestine charcoal suspension pushing rateThe results showed: After14days’ intragastric administration of thedrugs, the small intestine charcoal suspension pushing rate of model grouprats decreased obviously compared with that of normal group rats (P<0.05),while that of compound aloe capsule group, cisapride group, and Ma Zhi JiangZhuo group rats increased significantly (P<0.05). The small intestine charcoalsuspension pushing rate of treatment group rats was lower than that of thenormal group (P<0.05).4Morphological observation of intestinal pathologyThe observation of different intestinal tissues of the rats showed: thetissue structure was clear, mucosal was integral, there was no obviouscongestion, edema, inflammatory cell infiltration, erosion and ulcers and otherabnormality.5Expression of5-HT in intestinal tissuesThe results showed: After14days’ intragastric administration of thedrugs, the content of5-HT in the small intestine of the model group rats washigher than that of the normal group (P<0.05), while that of compound aloecapsule group, cisapride group, and Ma Zhi Jiang Zhuo group rats decreasedcompared with the model group (P<0.05). The content of5-HT in the smallintestine of treatment group rats increased compared with the normal group(P<0.05), but there was no significant difference between treatment groups(P>0.05).In ileocecus: The content of5-HT in the intestinal tissue of the model group was higher than that of the normal group (P<0.05), complex compoundaloe capsule treated rats, cisapride treated rats, Ma Zhi Jiang Zhuo the5-HTcontent in rat intestinal tissue treatment group compared with the model group(P<0.05),5-HT content of rat intestinal tissue in each treatment groupcompared with higher than normal group (P<0.05), between the treatmentgroups The content of5-HT in the intestinal tissue showed no significantdifference (P>0.05).Elevated from the anus in the colon (5cm):5-HT content in rat intestinaltissue model group than in normal group (P<0.05), compound aloe capsuletreated rats, cisapride-treated rats, the hemp Hovenia drop reduce the5-HTcontent in the the turbidity treatment group rat intestinal tissue compared withthe model group (P<0.05), and5-HT content of rat intestinal tissue in eachtreatment group compared with higher than normal group (P<0.05), in thetreatment groups5-HT content between intestinal tissue showed no significantdifference (P>0.05).Conclusion:1After the model of constipation-predominant irritable bowel syndromewas established successfully, stool shape, texture and water content of themodel rats changed obviously. After treatment, the rat stool became larger,softer, and the water content increased significantly.2Ma Zhi Jiang Zhuo recipe could increase the rate of small intestinepropulsion, improve the gastrointestinal motility in rats, thus played atherapeutic role for constipation. This may be one of the mechanisms of itstherapeutic effect for constipation-predominant irritable bowel syndrome.3Ma Zhi Jiang Zhuo recipe could reduce the expression of5-HT in therat intestinal tissues, which had similar functions to cisapride. This may bealso one of its mechanisms for treating constipation-predominant irritablebowel syndrome.4Although the condition of the rats in treatment groups eased obviouslycompared to model group, but still did not reach the standard of the normalgroup. So two weeks’ treatment was only effective time, we should appropriately prolong the therapeutic period in order to cure the syndrome. |
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