Methylation And Expression Of SOX17Gene In Endometnal Adenocarcinoma

Background and objectivesEndometrial cancer (EC) is in a group on the endometrial epithelial malignant tumors, during which endometrial adenocarcinoma is most common. In recent years, EC, its incidence is in rendering clear upward trend worldwide. Currently the pathogenesis of endometrial cancer is not very obvious, but many studies suggest that certain signaling conduction anomalies may be associated with EC development.SOX17was found in recent years a new tumor suppressor gene, P-chain effects is the Wnt signal transduction pathway in regulation of traditional Chinese medicine, involved in a variety of cellular signal transduction pathways, and closely related to tumor development. Our preliminary study has confirmed that gene Sox17, and P-catenin in endometrium cancer role and relevance in the process occurs, SOX17confirmed for research development of endometrial cancer is affecting.Epigenetics refers to changes based on in gene expression induced by non-gene sequences, is to study nucleotide sequences of the genes do not change case, gene expression can be a genetic branch discipline of genetic change.DNA methylation is under the action of the cytosine by a DNA methyltransferase in the genome CpG island dinucleotide5’ side carbon covalent bond with a methylation group.CpG island is located in a variety of vertebrates known gene transcription initiation sites around, cytosine (C) and guanine (G) composed of tandem repeats. Due to DNA methylation and the close relationship between human development and tumor diseases, particularly induced by CpG island methylation inactivation of tumor suppressor genes transcription problems, DNA methylation has become the Epigenetics and the apparent importance of genomics research, is research in recent years, SOX17endometrial carcinoma seen in gene methylation of reports.Therefore, the purpose of this study was to detect methylation situation and expression of SOX17in adenocarcinoma and normal endometrial tissue, to analyse relationship between clinical pathology and to explore the role and relevance of SOX17methylation in human endometrial carcinoma occurring, in order to provide a reference for genesis, development and treatment of EC.Research objects and methodsExperimental specimen:Collect surgical removal of fresh specimens from Gynecology of the Second Affiliated Hospital of Zhengzhou University from July2010to October2012, which were diagnosed by postoperative pathology of37adenocarcinoma cases,10normal endometrial tissue (from endometrium of uterus resection of uterine myoma or uterine adenomyosis) cases and5cases of peripheral blood of healthy persons.Experimental methods:1) The use of methylation-specific PCR (MSP) assay for detection of Sox17methylation.2) Immunohistochemistry (SP) assay for detection of SOX17expression in tissue specimens.Statistical methods:Statistical analysis of experimental data using SPSS17.0, groups analysed by χ2test, P<0.05, a=0.05as test standards.Results1.SOX17methylation status in endometrial adenocarcinomaSOX17gene methylation in30(81.08%) cases of endometrial carcinoma detected in specimens, of which19cases for complete methylation,11cases of partial methylation;1(10%) case of normal tissue methylation can be detected in a specimen, for part of the methylation. Group of endometrial adenocarcinoma and complex hyperplasia in endometrial tissue methylation compare the difference statistically significant (χ2=14.176,P<0.05).2.Relationship between methylation and clinicopathological parameters of SOX17gene in endometrial adenocarcinomaSOX17gene methylation status and patient age (P=0.635), TNM cancer staging (P=0.492), and lymph node status (P=0.281) relationship between clinical pathology parameters, such as there is no clear statistical significance. And tumor differentiation (P=0.004) and depth of myometrial invasion (P=0.015), a statistically obvious relationship.3.SOX17expression in endometrial adenocarcinomaSOX17on a positive expression of the proteins are mainly color rendered in the nucleus, leaving a few located in the cytoplasm or cell membrane. In37cases of endometrial adenocarcinoma positive rate of37.84%, of which12were for weak positive,2cases of positive expression.9in10normal endometrial tissue is strongly positive,1case for positive expression. Group with normal endometrial adenocarcinoma of endometrial SOX17expression for the group, the difference was statistically significant (χ2=9.795,P<0.05).Conclusions1. SOX17genes in endometrial adenocarcinoma high methylation status and low expression levels, prompting SOX17gene methylation may have been involved in the occurrence of endometrial adenocarcinoma;2.SOX17gene methylation status and age, FIGO staging in patients with lymph node metastases are not located with tumor differentiation and infiltration depth of grass-roots that prompts the methylation status of the gene testing may be for the prognosis of endometrial adenocarcinoma has a certain significance;3.SOX17endometrial adenocarcinomas occur in gene methylation is high during the event, and may be regulated SOX17important mechanisms of gene expression.