Experimental Study Of Meishen Capsule On Postmenopausal Osteoporosis

Objective:To study the effects of Meishen capsule on bone mineral density and bone Metabolism in osteoporosis rats induced by ovariectomy.Methods:60SD female rats were randomly divided into6groups, which were sham-operated group, osteoporosis model group, Gengnianxin capsule group, Meishen capsule with high dose, medium dose and low dose groups. Osteoporosis model was established by ovariectomy. The rats were treated consecutively for90days with Meishen capsule after10days of ovariectomy. The effects of Meishen capsule on Bone mineral density(BMD),Bone mineral content (BMC),Calcium(Ca),Phosphorus (P),Bone gamma-carboxyglutamic-acid-containing proteins(BGP),Alkaline phosphatase(ALP)and Tartrate-resistant acid phosphatase(TRAP).Results:(1) The variation of bone mineral density in ovariectmoized rats:compared with model group, the Meishen capsule group (6.4g/kg) has the significant difference in bone mineral density in lumbar vertebrae and right femur(p<0.05), Meishen capsule group (12.8g/kg) has the significant difference in bone mineral density in whole body、lumbar vertebrae and right femur (p<0.05or p<0.01).(2) The variation of bone mineral content in the right femur in ovariectmoized rats:compared with model group, the Meishen capsule group(6.4g/Kg) has the significant difference in the variation(p<0.05) and the Meishen capsule group(12.8g/kg) has the extremely significant difference in data(p<0.01).(3) The variation of bone Ca、p in the right femur of ovariectmoized rats:compared with model group, the Meishen capsule group(6.4g/Kg) has the significant difference in the variation(p<0.05) and the Meishen capsule group(12.8g/kg) has the extremely significant difference in data(p<0.01).(4)The variation of the content of Calcium in ovariectmoized rats:the Meishen capsule(12.8g/kg) has the significance difference compared with model group (p<0.05).(5) The variation of the content of Phosphorus in ovariectmoized rats:the Meishen capsule(12.8g/kg) has the extremely significance difference compared with model group(p<0.01).(6) The variation of the content of Bone gamma-carboxyglutamic-acid-containing proteins in ovariectmoized rats:the Meishen capsule(6.4g/kg and12.8g/kg) has the extremely significance difference compared with model group(p<0.01).(7) The variation of the content of Alkaline phosphatase in ovariectmoized rats: compared with model group, the Meishen capsule(6.4g/kg) has the significance difference (p<0.05) and the Meishen capsule(12.8g/kg) has the extremely significance difference (p<0.01).(8) The variation of the content of Tartrate-resistant acid phosphatase in serum of the ovariectmoized rats: the Meishen capsule(3.2g/kg) has the significance difference compared with model group(p<0.05) and the Meishen capsule(6.4g/kg and12.8g/kg) has the extremely significance difference in the results(p<0.01).All the results below demonstrated that the effective dose of Meishen capsule is between6.4g/kg and12.8g/kg, and the dose of12.8g/kg has the significant effective result. Conclusion:The Meishen capsule group(6.4g/kg and12.8g/kg) have the effect to increase the content of BMD and BMC, also can decrease the content of Ca,P,BGP,ALP and TRAP in serum in ovariectmoized rats. The Meishen capsule seemed had the effect in osteoporosis in ovariectmoized rats.