| Background:Granulocyte colony-stimulating factor (G-CSF) is a protein that stimulates differentiation, proliferation, and survival of cells in the granulocytic lineage. Recently, a neuro protective effect of G-CSF was reported in a model of cerebral infarction and a model of spinal cord injury (SCI).Aim:To investigate the possibility of Granulocyte-Colony Stimulating(G-CSF) treat spinal cord injury therapy by observing beneficial effects of G-CSF on motor function recovery after spinal cord injury in rats. Methods:50adult Wistar rats,4of them received only the laminectomy but no impact (n=4). The rest46animals were subjected to the spinal cord injury model at T10section using improved Allen’s weight drop method. Randomly divided the rest46animals into vehicle group (n=23) and G-CSF group (n=23), G-CSF group were treated post-operatively with G-CSF, and vehicle group use PBS.The animals’motor behavior was evaluated with Basso-Beattie-Bresnahan score (BBB score)and modified Rivlin loxotic plate test monitored weekly for5weeks postinjury (day1,7,14,21,28and35),and Grid walk test from2nd week for4weeks post-operatively(day7,14,21,28and35).Results:Seven animals died before the observation period for trauma or infection,three in G-CSF group and four in vehicle group, the rest were survived till the observation ended.Hind limbs paralysis occurred in all animals postoperatively.There are significant differences between two groups with a positive influence of G-CSF treatment on the motor behavior test(p<0.05at day7and p<0.01at day14,21,28,35of mean BBB score. p<0.05at day14and p<0.01at day21,28,35of Mean Grid walk test errors. p<0.01at day7,14,21,28and35of Mean modified Rivlin loxotic plate degree). Conclusion:Consulted all SCI studies which report improved functional recovery following G-CSF therapy,and together with the results of this experiment, G-CSF is play a positive influence of treatment on the motor functional recovery. |
PDF Full Text Request